Overview Non-Hardening Mountants Selection Guide


Glycerol Based n


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AF1 A glycerol-PBS solution contains an amine antifadent.


AF1 plus DAPI contains both an amine antifadent and the DNA stain, DAPI.


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AF2 A glycerol solution contains an amine antifadent that allows you to choose your own buffer solution.


n AF4 A glycerol solution of n-propyl gallate. High Refractive Index Glycerol Based Solutions


These solutions have a refractive index that matches that of the glass of the coverslip thereby minimizing the effects of spherical aberration. Such mountants are invaluable for imaging the internal structures of samples by techniques such as single multi-photon confocal niche since it acts as an efficient Clearing Solution (clearing occurs within a few minutes – Sean Speese, Oregon Health and State University, Personal Communication). The beneficial effects of these reagents can be seen in the two figures below (see page 10) where it can be seen that high resolution images have been obtained at a significant depth within the mountant solution. Additional features of these solutions include their total water miscibility, they are non-odorous and do not quench the fluorescence of fluorochromes (unlike thiodiethanol, another proposed high refractive index mountant) and the solutions are stable for long periods (at least 1 year).


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CFM-1 is a glycerol-PBS buffered solution having a refractive index of ~1.52 which can be used for transmission microscopy as well as with epifluorescence microscopy.


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CFM-1 plus AF is a glycerol-based solution having a refractive index of ~1.52 (at room temperature) that contains an amine antifadent


Citifluor Non-Hardening Antifadents High Refractive


Glycerol Based Index AF1


CFM-1


AF1 + DAPI AF2


AF4


CFM1 + AF CFM-2 CFMR2 CFM-3


* P = HDPE plastic bottle, G = Brown glass bottle ** Please ask for a quotation for larger volumes


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www.emsdiasum.com www.citifluor.com


AF3 AFR3 n


CFM-2 is a glycerol-tris amine buffered solution having a refractive index of ~1.52 (at room temperature) and a pH of ~8.5


CFM-3 – This glycerol-based antifadent contains a phoenlic antifadent of neutral pH and a refractive index of ~1.52 which also acts as a Clearing Solution enabling visualization of fluorochromes deep within the sample.


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CFMR2 – This product was designed for use with samples labeled with GFP. It contains a unique antifadent which does not de-oxygenate the solution which is an essential property if the GFP is not to bleach.


Glycerol Free n


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AF3 is a PBS solution containing an amine-based antifadent.


CFPVOH is an aqueous solution of poly (vinyl alcohol). Provided care is taken not to allow the water to evaporate, it will remain fluid. Retardation of photobleaching is effected by the addition of the aqueous antifadent solution AF100.


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AF100 is a PBS solution of an amine-based antifadent that if used as an additive to CFPVOH (1 part AF100 to 9 parts CFPVOH). Provided care is taken not to allow the water to evaporate, it will remain fluid.


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AFR3 is a PBS solution containing a NEW non- amine, non phenolic antifadent.


High Refractive Index Glycerol Free Solutions n


AF87 is an immersion oil having a refractive index of 1.52 and contains an antifadent. It may be used as an immersion oil and also as a mountant. Since AF87 is an oil that is immiscible with water, it is essential that specimens are dehydrated before application of the mountant.


Glycerol Free Index CFPVOH + AF100 AF87


High Refractive


Glycerol-Based Anatidae Reagents


pH RI P/G*


AF1 ~9 1.463 P AF2 NA 1.473 P AF4 NA 1.476 G


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