Hardening Mountant Solutions Glycerol-Based Solutions


These are systems with the antifadents being contained in the AF200 and AF300 solutions. These antifadents may be used with the aqueous polymer systems MWL 4-88 and CFPVOH.


n Citifluor AF200 Mountant Solution Usage


AF200 is a solution of and antifadent in glycerol and has been specially prepared for use with MWL 4-88 solutions where DAPI is the staining material. The solutions may be used with other fluorochromes such as fluorescein, Alexa dyes and Hoechst dyes. The presence of the antifadent helps to reduce photobleaching of the fluorophore. Solutions should be made up by mixing 1 part by volume of AF200 with 3 parts by volume of MWL 4-88. These mixtures should be used within 10 hours as the efficacy of the antifadent reduces with time. A few drops of the solution should be applied to the specimen followed by a cover slip. When used with MWL 4-88, evaporation of the water leaves a clear film which holds the cover slip in place, thereby aiding the safe storage of the specimen.


Properties and Storage


The solution is of medium viscosity and has a water-white appearance. The AF200 / MWL 4-88 solutions do not keep and the efficacy of the antifadent is markedly reduced over a 10 hour period. With this in mind, it is better to make up the mixture prior to use and not to rely on keeping solutions.


Cat No. RT 17977-10 Description Citifluor AF200


n Citifluor AF300 Mountant Solution Usage


AF300 a solution of and antifadent in glycerol and has been specially prepared for use with MWL 4-88 solutions where fluorescein is the staining material. The solutions may be used with other fluorochromes such as rhodamines, etc. The presence of the antifadent helps to reduce photobleaching of the fluorophore and to enhance the rate of solidification. Solutions should be made up by mixing 1 part by volume of AF300 with 3 parts by volume of MWL 4-88. A few drops of the solution should be applied to the specimen followed by a cover slip.


Properties and Storage


The solution is of medium viscosity and has a water-white appearance. The AF200 / MWL 4-88 solutions do not keep and the efficacy of the antifadent is markedly reduced over a 10 hour period. With this in mind, it is better to make up the mixture prior to use and not to rely on keeping solutions.


Cat No. RT 17977-25 Description Citifluor AF300 Qty. 10 ml Qty. 10 ml


16


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