Overview Choosing the Best Antifadent For Your Application
These are the critical decision factors which will help you select the most appropriate mountant: Do you need a Hardening or a Non Hardening medium? If your system is Glycerol tolerant than choose a Glycerol-Based product, if not then use a Glycerol-Free solution.
These high refractive index mountant solutions are to be used where you wish to obviate the effects of spherical aberration (caused by a mismatch between the refractive index of the glass of the coverslip and the mountant medium) which leads to a loss of resolution of your images.
The table below illustrates the main types of antifadent available. Glycerol-Based Mountants
Non-Hardening Mountants
AF1 AF1 Plus DAPI AF2 AF4 AFP1
Hardening Mountants
Tris MWL 4-88 + AF200
Tris MWL 4-88 + AF300
CFPVOH + AF200 CFPVOH + AF300
If you are unsure about which mountant to use, please don't hesitate to contact us, either by phone or email. We have technical staff on hand who are happy to answer the simplest, or the most challenging of questions.
Many of our products are designed for routine applications where consistent quality and low cost are essentials. The fact that many pathology laboratories place standing orders for large volumes is testimony to the quality and the “value for money” afforded by these products. For users ordering AF1 solution in bulk the cost is much lower. No other commercially available antifadent matches this price thus making us undoubtedly the most cost effective supplier of anti-bleaching agents.
All our solutions and reagents are prepared by a qualified (Chartered) chemist.
All our solutions are prepared in the US.
Our High Refractive Index mountants are unique. There are no other commercially available mountant solutions having a refractive index which match glass and enable visualization at such depths within a sample.
Electron Microscopy Sciences In PA: (215) 412-8400 • Toll-Free (800) 523-5874 Fax (215) 412-8450 or 8452 • email:
sgkcck@aol.com or
stacie@ems-secure.com •
www.emsdiasum.com
3 HIGH REFRACTIVE INDEX
CFM-1 CFM-2 CFMR-2 CFM-3 CFM-1 Plus AF
Glycerol-Free Mountants
CFPVOH Plus AF100
AF3 AFR3
HIGH REFRACTIVE INDEX AF87
PVP + Antifadent
CFPVOH + Antifadent
CFPVOH Plus AF100
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