Non-Hardening Mountant Solutions Glycerol-Free Solutions


n Citifluor AF3 Mountant Solution Usage


AF3 is a mountant solution composed of phosphate buffered saline and an antifadent. It was specifically designed to stop the photobleaching of the fluorescein moiety of FITC labelled biological specimens. Its application is not however limited to FITC labelled materials and has been used with advantage with many other fluorochromes including rhodamines, Alexa dyes and DAPI. It is useful for examining tissue live cells although it should be noted that cell lysis will take place over a period of time. If this is a problem, use our AFR3 solution.


The solution has a pH of ~10. The solution should be pipetted onto the specimen and then a cover slip applied. Specimens mounted in AF3 solutions have been kept in this way for many months without suffering damage.


Properties and Storage


The solutions have a water-white in appearance. They may be stored at room temperature and ideally between 50 and 150 and out of strong sunlight. The cap of the bottle or if using the pipette supplied with the material, the cap which covers the pipette delivery point, should always be replaced after use as a matter of good practice. Samples stored under these conditions for 6 months have shown no apparent deterioration. If the AF3 solution is being used in an assay, a control experiment should always be carried out.


Cat No.


RT RT


17972-25 17972-100


Description Citifluor AF3 Citifluor AF3


n Citifluor AFR3 Mountant Solution


A new antifadent solution for use with live cells The imaging of live cells in conjunction with an antifadent poses problems. The classical antifadents, amines and phenols can interact with the cell surface leading to lysis. The new solution contains an antifadent at a much lower concentration than that used is in the currently available solutions and is designed to show minimal phototoxicity. Unlike other antifadents it does not de- oxygenate the solution. The antifadent is supplied in a phosphate buffered saline solution. Texas Red, Alexa and Cyanine dyes have been stabilized with the new antifadent. This solution has a refractive index of 1.34 and hence is ideally suited for use with Total Internal Reflectance Microscopy (TIRFM).


Cat No. RT 17973-10 Description Citifluor AFR3 Qty. 10 ml Qty. 25 ml 100 ml


14


www.emsdiasum.com www.citifluor.com


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