Non-Hardening Mountant Solutions Glycerol-Based, High Refractive Index Solutions


n Citifluor CFM-2 Mountant Solution Usage


This glycerol / tris buffered based solution has been specially formulated so as to have a refractive index of ~1.52 (at room temperature) which should be a close match to the refractive index of the glass of the objective lens and cover- slip. The solution has a pH of ~8.5 which is appropriate for fluorescein conjugates. The CFM solutions will be particularly useful for high magnification work where immersion oils are use to minimise distortion of the image due to refraction of the viewing light. The solutions should also be particularly valuable where three-dimensional imaging of specimens is carried out using confocal microscopy.


A few drops of the solution should be applied to the specimen followed by a cover slip.


Properties and Storage


The solution is of medium viscosity, having a water-white in appearance and a pH of ~8.5 It may be stored at room temperature. The cap of the bottles should always be replaced after use to prevent evaporation of water. The solution should be stored at room temperature and out of strong sunlight. Under these conditions solutions have been found to exhibit little apparent deterioration over a 6 month period.


Cat No.


RT RT


17982-10 17982-25


Description


Citifluor CFM-2 Citifluor CFM-2


n Citifluor CFMR2 Mountant Solution


A high refractive index mountant specially designed for samples labeled with GFP


The CitiFluor™ CFM series have proved very useful for imaging materials using CLSM. Samples labeled with GFP pose a special problem since the retention of a good image during imaging and upon storage is dependent upon the mountant solution containing oxygen. The classical antifadents consume oxygen when the fluorochromes are irradiated and in the case of GFP this accelerates the loss of the image.


CFMR2 contains a unique antifadent that does not de-oxygenate solution but nevertheless, affords protection to the GFP.


As with the other CitiFluor™ CFM solutions the mountant has a similar refractive index to the biological tissue and hence enables a good depth of viewing within the sample.


Cat No. RT 17979-10 12 Description Citifluor CFMR2 Qty. 10 ml


www.emsdiasum.com www.citifluor.com


Qty.


10 ml 25 ml


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