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Exemption of some substances - With MM > 800 g/mol or a molecular diameter > 1,5 nm


-


Polymer with a molecular weight fraction below 1000 g/mol < 1%,


- With water solubility < 10 µg/l Determination of alga growth inhibition (OECD 201)


• Detrmination of the concentration that causes a 50% reduction in growth rate compared with control cultures conducted in identical conditions.


• 72h at 23°C. • Measurement of cell concentration (microscope; colourimeter, particle counter, turbidity meter.


Scedesmus subspicatus


The following scheme represents WAF preparation.


• Validity: Control cell concentration x16in 72h and max pH variation 1.5 • Result: CI50 and CSEO.


In case of self-assessment by the applicant, for each substance, main component or for the hydraulic fluid, the applicant shall provide the test reports or literature data including the references demonstrating the compliance with the requirements.


No documents need to be submitted for substances or main components already registered in LuSC-list.


References [1] Technical Report 3.0 – Draft criteria proposal for revision of EU Ecolabel criteria – JRC.


[2] ISO 15380 - Lubricants, industrial oils and related products (class L) -- Family H (Hydraulic systems) – Specifications for categories HETG, HEPG, HEES and HEPR.


[3] Lubes magazine N° 126 “How to choose the most appropriate ultimate biodegradability test method”.


Test description


The two next illustrations describe the acute toxicity test on daphnia and on algae according to OECD guidelines.


Determination of daphnia immobilisation (OECD 202)


Daphnia Magna


• Detrmination of the concentration that causes a 50% immobilisation of the daphnia in the test within 48 hours.


• 48h at 20°C. • Measurement of the quantity of immobilised daphnia.


• Validity: - 2mg O2/l - Max 10% daphnia immobilised (control) - Reference (K2Cr207) located between 0.6 and 1.7 mg/l. • Result: CE50 and CSEO.


[4] ISO 12922 - Lubricants, industrial oils and related products (class L) -- Family H (Hydraulic systems) -- Specifications for hydraulic fluids in categories HFAE, HFAS, HFB, HFC, HFDR and HFDU.


[5] ISO, DIN, ASTM, OECD test methods and specifications.


Part 2 will appear as a Lube Digital Exclusive in January 2019.


For more information contact: Mr. Vincent Bouillon


vincent.bouillon@bfblab.com


LINK www.bfb-labs.com


LUBE MAGAZINE NO.148 DECEMBER 2018


27


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