Yeast fractions can mitigate the risk of zearalenone
Yeast fractions have shown to be an effective solution to minimise the negative effects of certain mycotoxins. What makes YCW’s effective as mycotoxin adsorbents?
BY VIRGINIE MARQUIS, PHD, TOXICOLOGY AND PATHOGENS R&D MANAGER, PHILEO BY LESAFFRE
T In vitro single
concentration study In vitro isotherm study
he global impact of mycotoxins is a constant food and feed safety issue, creating hazards which result in economic losses in farm animal production. Effective decontamination strategies have been
developed to counter this problem, with the most used methods involving the addition of mycotoxin detoxifiers to
Table 1 – Efficacy of Safwall in adsorbing ZEA. Conditions
Incubation at 37°C, pH3 and pH7, 1ppm ZEA
Incubation at 37°C, pH 3 or 7, ZEA concentrations ranging from 100ppb to 20ppm.
feed. As part of this process, mycotoxins are physically bound by binding agent which decreases the gastrointestinal absorption of toxins. In addition to their efficiency as postbiotics, delivering probiotic effects on microbiota and immunity, yeast cell walls (YCW’s) are widely used by the animal feed industry as mycotoxin adsorbents. Several studies have shown that most YCW’s, when tested in vitro, display evident mycotoxin reducing effects, but with differing levels of consistency. Our focus, therefore, is directed towards the mechanism of mycotoxin adsorption and what makes YCW’s an efficient mycotoxin adsorbent.
Mechanism of mycotoxin adsorption The interactive mechanism between yeasts and mycotoxins is complex, being determined by the structure and composition
Results 76% adsorption at pH3 80% adsorption at pH7.
2,5 3
1,5 2
0,5 1
0 0 1 2 3 Ceq (mg/L)
In vitro conditions simulating GIT
Incubation at 39°C, one step mimicking the stomach at pH 2.5 followed by intestine at pH 6.5, 1ppm ZEA.
In vivo toxicokinetic study Cross-over design, ZEA (5mg/kg BW) administered orally with or in broiler chickens
In vivo trial in gilts
without the YCW (0.2 or 0.5g/kg BW). Plasma concentrations of ZEA and metabolites quantified by LC-MS.
Trial period of 28 days. 600µg ZEA/kg feed. Parameters measured: vulva size, organs weight, ZEA and metabolites content in liver.
Isotherm of ZEA adsorption on different YCW’s (different composition, strain, origin, and structure). 78% adsorption.
Bioavailability reduced by more than 90% for 0.5g/kg BW Safwall dosage and by 60% for the dose 0.2g/kg BW.
Vulva size and relative weight of reproductive organs increased due to ZEA but
decreased to control value with Safwall addition. ZEA, α-ZEL and β-ZEL content decreased in liver in presence of Safwall compared to ZEA treatment alone.
40 ▶ MYCOTOXINS | NOVEMBER 2021 4 5 6 Safwall
Qeq(mg/g)
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80
