several kits. In addition, when it comes to aflatoxin M1 re- search in milk, it is worth noting that no sample manipula- tion is required, as bovine, goat and sheep milk can be run directly in the assay with their natural fat content. For milk screening, I’screen AFLA M1 and I’screen AFLA M1 milk are widely used in the dairy industry, service laboratories and official control bodies in the Mediterranean Area. The kits show a high correlation to HPLC and very high precision, selectivity and robustness, together with the fact that milk is analysed either through a quick centrifugation step (as per the ISO 14675 guideline) or directly with its natural fat content. Moreover, time-to-result is very low; up to 40 samples run in little more than one hour. I’screen AFLA M1 milk has been granted AOAC Research Institute Performance Tested Methods status (AOAC Cert. No. 072002) for use with raw bovine whole milk, skimmed and powdered milk. Alongside the B ZERO and I’screen line, the Celer test kits are available for detecting a broad range of mycotoxins, and come with five standards, single-well implementation, and an assay time of 15-20 minutes. The line is characterised by a shared sample preparation in water-methanol for all mycotoxins in cereals. As an alternative, H2AFLA and H2DON are solvent-free, fast analytical solutions for total aflatoxins and deoxynivalenol respectively (Table 1).
Automation to improve lab efficiency Automated ELISA processing saves significant work time in the laboratory and delivers accurate results, which is especially important during high-volume periods. Eurofins Tecna strives to provide industries with fully automated methods for ELISAs implementation. Most kits are validated for the Bolt, a compact, flexible, easy-to-use, walk-away automatic analyser from sister company Gold Standard Diagnostics. The Bolt supports all the ELISA processing steps in a fully walk-away mode, releasing an automatic report, supported by several curve algorithms. The strict validation protocol eliminates all potential errors. Field application teams can install a Bolt unit and get the analysis started in around three hours, with no need for on-site method adjustment or validation. Automatised processes are an advantage for low-to-medium analytical volumes, especially for mix-and-match sessions, as the instrument can autonomously run up to four compatible methods of the same line in one frame, without mismatching reagents or getting stuck in multiple parallel sessions. The same robot can potentially analyse any kind of ELISAs for different targets and is already validated for the analysis of antibiotic residues in foods of animal origin, food pathogens, allergens, and glyphosate.
▶ MYCOTOXINS | NOVEMBER 2021
Automated ELISA processing saves significant work time in the laboratory and delivers accurate results.
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