MICROSCOPY & IMAGING


In 2013, researchers used cryo-EM


to view the structure of a tubular HIV-1 capsid-protein assembly at 8Å resolution, and cryo-ET to recreate a 3D structure of a native HIV-1 core. Teir work paves the way for further studies of capsid function and for targeted pharmacological intervention. More recently, cryo-EM has shown the structure of Cyclophilin A in complex with the assembled HIV-1 capsid at 8 Å resolution, offering new insights into how the protein stabilises the HIV-1 capsid and is recruited to facilitate HIV-1 infection. Te next steps in this research are combining cryo focused ion beam microscopy (cryo-FIB) and cryoET in situ investigations of virus-infected cells.


STRUCTURAL BIOLOGY AND DRUG DESIGN Dave Stuart is Diamond’s director of Life Sciences, and a world leader in Structural Biology. His research has


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up orders of magnitude. He says, “It’s speeded up with automation – now you can respond quickly enough in terms of providing structural information to hook directly into pipelines for developing new chemistries. So I think it’s had a fundamental change in the way people think about drug discovery.


Te driver for setting up eBIC was to do the same thing for cryo-EM/ET; to provide a centre with enough critical mass to be efficient, to help to try and develop the methods to try and develop the throughput of electron microscopy. If you collect X-ray diffraction data at Diamond, the software will automatically


Nobel Laureate Richard Henderson and Diamond CEO Andrew Harrison at the eBIC official opening in 2018


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