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MICROPLATE READERS


LOW-ADHESION PLATES FACILITATE ORGANOID CULTURE A


MS Bio reports on how several leading European research groups have used its Lipidure coated


microplates to help culture physiologically functional, light-responsive retinal organoids. Development of in vitro models of the human retina in which to perform pharmacological and toxicological studies is an urgent area of vital research. An essential step for developing in vitro models of human retina is the ability to generate laminated, physiologically functional, and light responsive retinal organoids from renewable and patient-specific sources. Professor Majlinda Lako from the


Institute for Genetic Medicine at Newcastle University, UK commented, “The Lipidure- coated plates provided by AMS Bio were extremely useful for generating with ease large numbers of homogeneous retinal organoids that responded to light and contained all the key retinal cell types.” Organoids are organ-like structures that can be formed by 3D cell culture and differentiation of stem cells or organ progenitors; and are capable of recapitulating aspects of organ function in


vitro. Retinal researchers at the Institute for Genetic Medicine at Newcastle University (UK) and New Cells Biotech have achieved this by using low-adhesion cell culture conditions. Lipidure-coated plates use MPC


(2-methacryloyloxtethyl phosphorylcholine), a biocompatible synthetic polymer with the same structure as the phosphorylcholine polar bases that form cell membranes. As a consequence of the high biocompatibility of this coating, cells will not adhere to the surface of Lipidure-coated plates or dishes. Different well-shapes (U, V and flat-bottom) can be used to control size, position and the density of organoid culture. Studies have


shown that these coated plates promote the formation of a single spheroid in concave (U or V-shaped) wells, while other brands of plate lead to formation of satellite spheroids - indicating a higher degree of cell adhesion is taking place. Formation of spheroids, tumour spheres,


embryoid bodies and neurospheres using these plates have been demonstrated for cell types including ES and iPS (human and mouse), NIH3T3, pre-adipocytes, HepG2 and other cancer cell lines as well as primary neuronal cells.


For more information visit www.amsbio.com


GLASS-BOTTOM MICROPLATES for IMAGING APPLICATIONS The automation-compatible Krystal


he latest family of ultra-flat Krystal glass bottom microplates developed by Porvair Sciences provide excellent performance for high- throughput screening and tissue culture applications.


T


plates combine the advantageous optical properties of glass, low background and low birefringence, with the versatility of a microplate. Precision engineered, using a polystyrene frame and a borosilicate glass sheet fixed to the base with a biocompatible adhesive, Krystal plates offer a high degree of planar flatness (+/- 50 microns) across the base, excellent light transmission and a flat optical plane for growing cells. In addition,


the nominal cut-off wavelength of 335nm allows most fluorescence assays to be excited or read through the plate glass bottom. These advantages together also


translate into a significant increase in measurement precision and elimination of read errors when performing cell-based assays using fluorescent or luminescent imaging. These glass bottom plates are proven


to demonstrate higher performance than standard polystyrene plates for whole- plate CCD imaging, laser detection and high-resolution microscopy using confocal imaging. They are available in a choice of ANSI/SLAS standard 96- and 384-well formats.


For more information visit www.porvair-sciences.com


www.scientistlive.com 21


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