45 Sample Preparation & Processing
Summary
The pursuit of understanding and combating multiple myeloma through bone marrow research has gained significant momentum in the last few years. However, the complexity of bone marrow continues to hinder researchers’ abilities to extract an ample supply of cells from samples, and the subpar preprocessing steps in place to overcome this are time consuming and tiresome. Fortunately, the landscape is evolving and the introduction of innovative immunomagnetic technology is able to sidestep these preprocessing bottlenecks. By eliminating these obstacles, this cutting-edge solution is proving instrumental in enhancing the efficiency and efficacy of multiple myeloma research, ultimately propelling scientific discovery in this critical field.
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Optimising tube automation for maximum sample recovery
Azenta Life Sciences has unveiled its ground-breaking tri-coded 1.6ml Maximum Recovery tube, specifically designed for automated liquid handling workflows. The tube’s distinctive shape at the bottom enhances sample retrieval, making it an ideal
Addressing the challenge of large dead volumes in storage tubes, particularly crucial for handling precious samples such as stem cells and enzymes, Azenta Life Sciences emphasises the limitations of manual pipetting in high-throughput labs relying on automated liquid handling systems.
To optimise sample recovery, Azenta’s research underscores the importance of addressing factors contributing to dead volume in storage tubes. The incorporation of internal compound-curve tapers near the bottom of the tube is identified as a key design element. This innovation reduces the total volume stored in the critical area while maintaining accessibility for industry-standard pipette tips.
Comparative research between the new 1.6ml Maximum Recovery tube and standard 1.9ml tubes reveals a substantial advantage. Over a run of 100 tubes, the 1.6ml Maximum Recovery tube recovers an additional 16ml. This improvement translates into significant cost savings, especially with expensive reagents or valuable samples.
Beyond maximising sample recovery, the unique design of the 1.6ml Maximum Recovery tube aligns with sustainable practices, offering users an opportunity to reduce reagent purchasing costs and contribute to environmentally conscious lab workflows throughout the supply chain.
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ilmt.co/PL/kARd 61612pr@reply-direct.com Scaling-up iPS spheroid culture production
AMSBIO introduces the ABLE 3D Magnetic Stir and Disposable Bioreactor System, a cost-effective solution for lab-scale induced pluripotent stem (iPS) cell spheroid culture production.
Growing iPS cells in 3D spheroid suspension culture mimics the natural structure of embryoid bodies formed by embryonic stem cells. The ABLE 3D Bioreactor offers a user-friendly and economical tool that delivers high yield, viability, and efficiency for expanding human iPS stem cells and promoting differentiation.
Constructed from high-density polycarbonate for compatibility with iPS stem cell cultivation, the Bioreactor arrives ready-to-use for non-adherent cell growth, eliminating the need for costly extracellular matrix (ECM) proteins to coat plasticware. Its delta-wing-shaped impeller, featuring a magnet on each blade, creates low shear agitation through laminar flow, facilitating the development of uniform 200-300 μm spheroid cell clusters.
The bioreactor system employs disposable vessels for cell culture or production, yielding up to 5 × 107 cells per 30 mL vessel, equivalent to the cell output from ten 10 cm culture dishes or ten 6-well plates.
Seamlessly integrating with StemFit media, a chemically defined stem cell culture medium, the ABLE 3D Bioreactor supports the efficient maintenance of induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) without the need for feeder cells. By using iMatrix-511-coated plates along with StemFit media, human iPSCs can be readily harvested, dissociated into single cells, and seamlessly transitioned into the ABLE 3D Bioreactor system for large-scale and efficient iPSC spheroid culture.
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ilmt.co/PL/KeNV 61459pr@reply-direct.com Create rapid conditions in anaerobic jars
Don Whitley Scientific (DWS) has announced the launch of a new gassing system for the rapid generation of multiple atmospheres (anaerobic, microaerobic and capnophilic) in gas jars.
The Whitley AtmoGen - the new Jar Gassing System - has fresh, modern graphics, bespoke software, and optional dual gas input. AtmoGen can be used to gas three separate jars simultaneously with the same atmosphere.
Joe Walton, Director at DWS, explained the cost saving figures: “We have calculated that, if you are processing 30 samples per day, the running cost of AtmoGen is less than £600 per year whilst using gas packs costs £3,580 per year. Based on this, the cost of the AtmoGen could be recouped in less than two years.”
The system is easy to use, with no complicated training required, and can be operated with other, non-DWS anaerobic jars (with an adaptor kit). It also features a new catalyst check function to ensure that an effective catalyst is in place before incubation. AtmoGen has a 7 inch full colour touchscreen,
USB and Ethernet ports, data logging, and comes complete with one Whitley Incubation Box. More information online:
ilmt.co/PL/bVD0
61833pr@reply-direct.com
References
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2. Dean L. Blood Groups and Red Cell Antigens [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2005. Chapter 1, Blood and the cells it contains. Available from:
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nih.gov/books/NBK2263/
3. Wang, H., Leng, Y., & Gong, Y. (2018). Bone marrow fat and hematopoiesis. Frontiers in endocrinology, 9, 694. 4. Fazeli, P. K., Horowitz, M. C., MacDougald, O. A., Scheller, E. L., Rodeheffer, M. S., Rosen, C. J., & Klibanski, A. (2013). Marrow fat and bone—new perspectives. The Journal of Clinical Endocrinology & Metabolism, 98(3), 935-945.
5. Ye, H., Adane, B., Khan, N., Sullivan, T., Minhajuddin, M., Gasparetto, M., ... & Jordan, C. T. (2016). Leukemic stem cells evade chemotherapy by metabolic adaptation to an adipose tissue niche. Cell stem cell, 19(1), 23-37.
6. Bai, Y., & Chim, C. S. (2019). Ficolled bone marrow is superior to bone marrow buffy coat for detection of minimal residual disease in multiple myeloma. Hematology, 24(1), 533-537.
7. Pösel C, Möller K, Fröhlich W, Schulz I, Boltze J, Wagner DC. Density gradient centrifugation compromises bone marrow mononuclear cell yield. PLoS One. 2012;7(12):e50293. doi: 10.1371/journal.pone.0050293. Epub 2012 Dec 6. PMID: 23236366; PMCID: PMC3516517.
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