29 Proteomics, Genomics & Microarrays Validated antibodies support stem cell purity and consistency
AMSBIO has extended its antibody portfolio to support quality assurance in stem cell research and therapeutic development. The new antibodies offer high specificity and batch-to-batch consistency for techniques including flow cytometry, immunocytochemistry, Western blotting and ELISA.
Among the latest additions are monoclonal antibodies targeting key pluripotency markers - SSEA-3 (Gb5), SSEA-4 (SialylGb5) and Lewis X (SSEA-1). These Stage-Specific Embryonic Antigens (SSEAs) are widely used to assess the undifferentiated state of human pluripotent stem cells and monitor early differentiation.
The antibodies are raised against glycan epitopes that are uniquely expressed on human stem cells, with minimal cross-reactivity to non- target glycans as confirmed by ELISA validation. This ensures accurate detection and reduces the risk of false positives in routine QC and research workflows.
Dr Philipp Boder, stem cell specialist at AMSBIO, said: “Whether for basic research or therapeutic development, antibody quality directly impacts reproducibility. Our rigorously validated antibodies help scientists confirm culture integrity and compare results across labs with confidence.” He added: “In regenerative medicine, this level of reliability is key to ensuring the safety and consistency of cell-based therapies.”
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ilmt.co/PL/NwOz 64869pr@reply-direct.com Multiomic sequencing assays advance methylation studies
EpiCypher, a leader in epigenomic technologies, has introduced two innovative DNA methylation sequencing assays - CUTANA® meCUT&RUN and Multiomic CUT&RUN - that offer scalable, high- resolution insights into gene regulation to accelerate drug discovery.
Building on their established CUT&RUN platform, which enables precise chromatin profiling from minimal input and reduces sequencing expenses, these new assays extend its capabilities to DNA methylation analysis. DNA methylation is a key epigenetic modification that controls gene expression and cellular identity, but traditional bisulphite sequencing methods often suffer from DNA damage, high costs, and large sample requirements, limiting their broader use.
The CUTANA® meCUT&RUN assay uses a novel methyl-DNA binding fusion protein to specifically enrich 5-methylcytosine (5mC) with base-pair precision. This method detects over 80% of methylated CpGs compared to whole-genome techniques while requiring more than 20 times less sequencing, offering a highly sensitive and affordable alternative for methylation mapping.
Expanding on this, the Multiomic CUT&RUN assay enables simultaneous profiling of DNA methylation alongside chromatin features such as histone modifications or DNA-binding proteins in a single experiment. This dual profiling reveals complex epigenetic interactions, providing valuable data for basic research and translational drug development.
Both assays are compatible with low cell inputs, including cell lines, primary cells, and patient-derived samples, and offer streamlined workflows suited for diverse research and drug discovery applications.
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ilmt.co/PL/wYED 64857pr@reply-direct.com ADVERTORIAL Scalable 6-well cell culture and fi ltration system for small molecule development
Porvair Sciences introduces the complete cultureware solution for design and scale up of small molecule development and production. Whether you’re working with recombinant proteins such as monoclonal antibodies or DNA products like plasmids, the Clara™ 6 Well Cell Culture plates are the perfect solution for transfection and growth of host strains. Coupled with the Clara™ 6 Well fi lter and receiver plate set to purify your molecule of choice, the Clara™ 6-well system has a simple high throughput hardware option for you. With customisable porous plastic fi ltration supports (polyethylene, polypropylene or PTFE in a wide range of pore sizes) and options for membranes (PES/PVDF/glass fi bre in 0.22 or 0.45 µm) the Clara™ 6-well system allows quick, simple and scalable purifi cation of a wide range of biological molecules.
Culture, express and purify product from up to 30 mL of mammalian, bacterial, yeast or plant cell line suspensions in multiwell format. All cell culture and fi lter plates are manufactured from virgin polystyrene ensuring high optical clarity, allowing easy observation of cell suspension optical density and fi ltration progress and effi ciency.
The dual grip ports of culture and fi lter plates enable automated handling by a wide range of robotic systems. With a standard SLAS/ANSI footprint, the Clara™ system is compatible with manual and automated incubators and deep vacuum manifolds making the plates ideal for both manual and automated methods. Unique plate barcoding allows sample tracking and traceability. Whatever your small molecule research and development the Clara™ 6 well system has a solution for your workfl ow.
More information online:
ilmt.co/PL/XwMA 64948pr@reply-direct.com
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