48 Buyers’ Guide 2021
Figure 5: Isocratic analysis of a CBD oil tincture (‘For the People’ 600 Orange). Column: Orosil C18-HC, 150x4.6- mm, 3 µm, 100 Å. Flow rate: 1 ml/min. Detection: 280 nm. Mobile phase: 25% 0.2% H3
PO4 with 75% ACN.
substituted for the tablet as the controller, in which case Clarity is then loaded onto the computer and the license transferred to it. The default mobile phase bottles have a 500-ml volume and fit inside the case. There is no reason why they have to be inside the case during operation, and some customers have ordered 2-L. bottles for the purpose instead. They must be carried separately when the unit is moved, a minor inconvenience. Add-ons such as a sample carousel and automatic sample injector would certainly compromise the portability of the device, as mentioned earlier, and would require operation using line current rather than the battery. However, if the machine is to remain in one location for an extended period, then such modifications would increase its capabilities to the point that they approach those of a conventional HPLC.
Future Applications
Figure 6: Isocratic separation of B-vitamins via HILIC. Column: PolyHYDROXYETHYL A, 200x4.6-mm, 5 µm, 60 Å. Flow rate: 1 ml/min. Detection: 255 nm. Mobile phase: 40 mM ammonium acetate, pH 5.0, with 75% ACN.
column usage is about US$ 1 per analysis. Figure 3 is a chromatogram of a composite sample of major haemoglobin variants.
B. Cannabinoids: This is one application where analysis in the field could be helpful. An example would be the analysis of extracts of cannabis from different locations at a farm to get more detail about differences in cannabinoid composition in the different places. This version of the machine is called SmartCanna LC™, with a detector wavelength of 280 nm. Figure 4 shows the gradient separation of a composite sample of cannabinoid standards.
Figure 5 shows an isocratic analysis of a CBD
oil tincture with an overlay of a composite mixture of cannabinoid standards.
Figure 6 shows the isocratic separation of B-vitamins in the hydrophilic interaction chromatography (HILIC) mode.
Custom Modifications
In addition to the wavelength of the detector module, the most common modification to date has involved the controller. It is somewhat cumbersome to enter data directly on the tablet that comes with the unit; a USB or Bluetooth keyboard and mouse make the task easy. A PC can be
Portable HPLC machines have not been available long enough to have entered markets which would truly exploit their portability. There are numerous application areas that would benefit from the technology, and move chromatography away from a laboratory based activity to one which is more inclusive of the population. One such niche would be analysis of water samples or fruit in the field in real time, rather than having to transport them back to a central lab. The results could inform the locations being sampled. As the technology in this area develops through the use of lighter components and higher-functioning separation and detection technology, the possibility of moving chromatographic analysis into the world at large becomes a potential reality. This would start to move the technology towards a future where separation science is seen as a means of ensuring a better lifestyle.
References
1. The Analytical Scientist 65 (June 2018) 26- 29: Dream Small.
2. V.M. Tulchinsky, D.E. St. Angelo. A Practical Portable HPLC System – MINICHROM, a New Generation for Field HPLC. Field Analyt. Chem. Technol. 2 (1998) 281-285.
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76
