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Figure 5. LC-MS compound chromatogram at different scaling, obtained in positive electrospray ionisation mode for a human plasma lipid extract. (a) and (b zoomed) 60 min gradient, (c) and (d zoomed) 120 min gradient.


shows the LC-MS compound chromatogram, demonstrating not only the complexity of the sample, but also the high separation efficiency obtained by the µ-pillar array column. The major lipid classes are nicely distinguishable within the chromatogram.


Conclusion


µ-Pillar array columns can offer tremendous steps forward in LC/MS applications. The novel approach of the structure allows for much more flexibility in the design of the separation channel, promising up- or downscaling of chromatographic methods. With the perfectly ordered positioning of the µ-pillars, and the tight control over the dimensions, much more homogeneous flow paths within the column are achievable, minimising peak broadening effects and improving injection to injection reproducibility. With the reduced back pressures, even at column lengths of 200 cm, column robustness can be increased significantly, allowing more sample injections, as demonstrated with the HeLa digest experiment.


With the development of products for increasing flow rate ranges, the µ-pillar array columns will continue to become available for more liquid chromatography applications. Currently, they are available for up to 15 µL/min, an inviting flow rate for


researchers working with limited sample amounts. But the promise of µ-pillars is likely to be upscaled to higher flow rates as well.


11. A. dell Campo and E. Arzt, Fabrication Approaches for Generating Complex Micro- and Nanopatterns on Polymeric Surfaces, Chem. Rev. 2008, 108, 911-945


Reference


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2. R.A. Henry, LC-GC vol 32, issue 4, 12-19


3. Thermo Scientific, https://analyteguru. com/revolutionizing-liquid-chromatography- with-the-vanquish-uhplc-platform/


4. Avantor, Chromatogr Today, May/June 2020, 26-28


5. B. He, et al, Anal Chem 70 (1998) 3790- 3797,


6. F.E. Regnier, J High Resol Chromatogr 23 (2000) 19-26


7. J. Op de Beeck, et al, J of Chrom A, 1239 (2012) 35-48


8. J. Op de Beek, et al, BioRxiv 2019, doi. org/10.1101/472134


9. M. Baca, et al, Anal Chem 2019, 91, 10932- 10936


10. W. De Malsche, et al, Realization of 1 x 106 Theoretical Plates in Liquid Chromatography Using Very Long Pillar Array Columns, Anal. Chem. 2012, 84, 1214 – 1219


12. W. De Malsche, et al, Experimental Study of Porous Silicon Shell Pillars under Retentive Conditions, Anal. Chem. 2008, 80, 5391-5400


13. M. Callewaert, et al, Integration of uniform porous shell layers in very long pillar array columns using electrochemical anodization for liquid chromatography, Analyst, 2014, 139, 618-625


14. Wei-Sheng Lei, et al., Die singulation technologies for advanced packaging: A critical review, J. Vac. Sci. Technol. B 30(4), Jul/Aug 2012


15. R.T. Kelly, Molecular & Cellular Proteomics August 26, 2020, https://doi. org/10.1074/mcp.R120.002234


16. J. Stadlmann, et al, Anal Chem 91 (2019), 14203-14207


17. PharmaFluidics Technical Note, https:// www.pharmafluidics.com/


18. K. Sandra, et al, Biopharmaceutical Perspectives, LC-GC Europe,Μarch 2018, 155-165


19. S. Kislyuk, et al, Anal Bioanal Chem 2018 Apr;410(11):2751-2764


20. K. Sandra, et al, Recent developments in HPLC and UHPLC, June 2017, 6-13


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