33


Figure 6. Comparison of chromatographic separation of protein mixture on four columns. (A) wide-pore fully porous FPP 1.7µm C4-400Å, (B) ultra-wide pore fully porous FPP 1.7µm C4-1000Å, (C) wide-pore superficially porous SPP 2.6µm C4-500Å, and (D) ultra-wide pore superficially porous SPP 2.6µm C4-1000Å columns. All columns have the same dimension 2.1mm ID x 50mm, and chromatographic condition: mobile phase A: 0.1% TFA in water & B: 0.075%TFA in 100% acetonitrile, gradient 20-70% B in 25min, temperature 60°C, flow rate 0.2ml/min, injection volume 5.0uL, UV 220nm


Table 3a. Peak information for FPP 1.7µm 400Å. Peak # tR(min)


NTP


1 2 3 4 5 6


10.312 25633 13.753 39455 16.934 14817 18.616 37091 20.745 28798 23.506 16896


Table 3b. Peak information for FPP 1.7µm 1000Å.


Resolution Symmetry factor N/A


12.906 7.654 3.577 4.875 4.567


Table 3c Peak information for SPP 2.6µm C4-500Å. Peak # tR(min) NTP


1 2 3 4 5 6


8.909 17846 N/A


12.401 27001 15.982 21959 16.967 41156 18.516 60190 21.020 59494


12.313 9.792 2.579 4.881 7.765


1.508 1.281 2.807 2.295 1.590 1.451


Resolution Symmetry factor 1.368 1.558 2.429 2.053 2.000 1.226


Peak # tR(min) NTP Resolution Symmetry factor 1 2 3 4 5 6


9.301 15599 N/A


12.898 31716 12.277 15.932 24431 8.724 17.394 51705 4.107 19.499 68888 7.001 21.516 65693 6.391


Table 3d. Peak information for SPP 2.6µm C4-1000Å. Peak # tR(min) NTP


1 2 3 4 5 6


9.751 29974 N/A


13.065 46978 16.392 28796 17.854 60225 19.468 55566 21.912 52631


14.245 10.634 4.328 5.210 6.880


0.835 1.469 2.811 1.862 2.007 1.733


Resolution Symmetry factor 1.838 1.821 2.420 2.138 2.113 2.181


Figure 7. Comparison of chromatographic separation of Cytochrome C on wide-pore fully porous FPP 1.7µm C4-400Å compared to the competitor 1.7µm C4-300Å column. Both columns have the same dimension 2.1mm ID x 50mm, and chromatographic condition: mobile phase A: 0.1% TFA in water & B: 0.075%TFA in 100% acetonitrile, gradient 20-70% B in 25min, temperature 60°C, flow rate 0.2ml/min, injection volume 5.0uL, UV 220nm.


Figure 8. Schematic representations of crystal structures of oxidised monomeric and oligomeric cyt c. (A) Structure of monomeric cyt c (PDB ID code 1HRC). (B) Structure of dimeric cyt c (red and blue).


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44  |  Page 45  |  Page 46  |  Page 47  |  Page 48  |  Page 49  |  Page 50  |  Page 51  |  Page 52  |  Page 53  |  Page 54  |  Page 55  |  Page 56  |  Page 57  |  Page 58  |  Page 59  |  Page 60  |  Page 61  |  Page 62  |  Page 63  |  Page 64  |  Page 65  |  Page 66  |  Page 67  |  Page 68  |  Page 69  |  Page 70  |  Page 71  |  Page 72  |  Page 73  |  Page 74  |  Page 75  |  Page 76