7 Luminescence,UV & Microplate Readers Second-Generation Polymer Fluorescent References Expands the Possibilities of Fluorescent Calibration
Starna fluorescent polymer references are well known and have been a de facto industry standard, dating back to the 1960s, for wavelength calibration and the monitoring of instrument performance in fluorescent applications. The development by Starna of second-generation polymer references based upon new proprietary dyes expand on this capability. With increased stability and resistance to photo-bleaching, the performance of these materials is extended to allow their use as relative photometric intensity references.
Development of these second-generation materials, specifically under Starna’s ISO 17034 accreditation, has ensured appropriate stability and homogeneity studies. This is in line with the essential requirements of this Standard, which have to be met for reference material production. These materials can therefore be used to qualify a wide variety of fluorescent instrumentation and provide traceability of measurement, facilitating comparison between measurements on a given instrument and between systems.
They are available in a variety of physical formats and concentrations, which may be optimised for specific instruments and applications. A range of concentrations further provide the possibility for linearity verification.
Starna offers a range of polymer, glass, and sintered formats as well as sealed liquid fluorescent reference materials to allow the testing of wavelength, spectral response, sensitivity and signal-to-noise. For more information, visit
www.starna.com/reference-materials.
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New Modular LED Light Source for Fluorescence Microscopy Launched
Bluebox Optics announced immediate availability of a new modular LED light source ideally suited for fluorescence microscopy.
“When speaking with researchers we identified the wish for a flexible and future-proof LED light source for single and multiband fluorescence microscopy. With the niji’s uniquely reconfigurable mainframe, LEDs and filters can be changed by the user in minutes, there’s no need to know today what wavelength you need in the future,” said Alan Hughes, Director of Bluebox Optics.
With up to seven LED channels the niji delivers powerful, multi-wavelength excitation from UV to Far Red. The niji allows users to start with one LED only, with its modular mainframe up to six more LEDs can be added at a later time, offering additional excitation centre wavelengths, excitation bands, and output power options as and when they are needed.
“With this new LED light source we enable our distribution partners to be able to offer a low cost entry point LED light source with a low cost upgrade path, making the investment very future-proof,” explained Alan Hughes.
The niji combines an impressive amount of features, both constant current and constant optical power LED drive modes are selectable, enabling fast imaging speed and high excitation power stability over long periods. Wavelengths, power, and emission channel can all be controlled individually or simultaneously, and the niji is fully compatible with most standard microscopes, MicroManager, MicroManager 2, and a wide range of other software packages. It is also a very quiet tool, which is much appreciated by users.
A datasheet is available for download at
www.blueboxoptics.com/niji.
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