5 Luminescence,UV & Microplate Readers Real Time Cell based Assays in a Microplate Reader


Cell-based assays have become an important application in the life science sector widely used in biotech companies and academic research institutions to monitor cell health in real-time.


Experiments undertaken with living cells require closely regulated physiological conditions including temperature and gas control. All BMG LABTECH microplate readers include precise temperature control as standard and the addition of the Atmospheric Control Unit (ACU) provides independent control of both oxygen and carbon dioxide for cell based assays.


BMG LABTECH’s Atmospheric Control Unit enables the independent control of both oxygen and carbon dioxide concentrations within the plate reader and replaces the requirement for manual intervention required for transferring plates between the incubator and the microplate reader, especially useful for kinetic assays saving time and labour and avoiding any temperature or atmospheric stress to the cells. It is the ideal solution for a wide range of cell bases assays, including long-term cell proliferation, hypoxia and cytotoxicity studies.


Available across the BMG LABTECH microplate reader range the Atmospheric Control Unit is accessible to all researchers whether using the single-mode Omega readers, LUMIstar®, SPECTROstar® and NEPHELOstar®, the multimode FLUOstar® Omega and POLARstar® Omega or the more sensitive and fl exible CLARIOstar® range.


The OMEGA multimode readers are perfect instruments for general life science laboratories providing an economic solution for multiplex assays including simultaneous reads of fl uorescence, absorbance and luminescence, ideal for measuring reporter gene expression whilst following cell growth and proliferation or cytotoxicity.


The more advanced features of the CLARIOstar and its associated Atmospheric Control Unit provides more opportunities to perform more elaborate assays with greater sensitivity.


Exclusive to the CLARIOstar Atmospheric Control Unit are unique features including tracking real- time gas values and gas ramping capabilities. Tracking the gas levels directly in the MARS data analysis software throughout the assay provides


retrospective confi rmation of the O2 and CO2 levels when analysing data. This provides confi rmation that the programmed gas and temperature conditions were maintained throughout and helps relate cells behaviour in relation to these conditions. The programmable controlled gas ramping provides for the fi rst time in a microplate reader the ability to perform ischaemia reperfusion type experiments that can be monitored in real time and related directly to the gas concentrations.


Ischaemia reperfusion assays reproduce the O2 changes cells experience with life-threatening conditions such as stroke, myocardial infarction or renal failure, whereby cells are temporarily deprived


of O2 and ischaemia - an essential nutrient. In-vitro experiments require an instrument capable of rapid deoxygenation, rapid reperfusion together with the parallel monitoring of critical biological parameters including cellular oxygenation and ROS, currently only possible on the BMG LABTECH CLARIOstar.


CLARIOstar® with ACU Full fl exibility in cellular assays with the new gas ramping function


For the fi rst time scientists are given the capability to fully regulate the environment within the microplate reader, by mimicking in vitro hypoxia, ischaemia/ reperfusion and much more.


The CLARIOstar with LVF MonochromatorsTM and CO2 is not only


the most-sensitive monochromator-based plate reader today, its Atmospheric Control Unit (ACU) can perform gas ramps, modifying O2


gas levels before rapidly restoring atmospheric conditions. Example of O2 with steady 5% CO2


20 18 16 14 12 10 8 6 4 2 0


0 Ischaemia Reperfusion O2


CO2


20


40


60


80 Time in minutes


deprivation and reoxygenation (down to 0.2% O2 (grey) performed by the CLARIOstar with ACU.


; purple)


100


120


The precise control of gases in all the BMG LABTECH microplate readers allows reproducible conditions to be programmed for all assays replicating physiological conditions pertinent to the cell line or tissue that you are studying.


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Gas concentration in %


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