27 A Robust Quantitative Alternative to the Scratch Assay
New independent research demonstrates that the Oris™ Cell Migration Assay presents a superior quantitative, high throughput alternative to the scratch assay. Cell migration is integral to many physiological processes, including embryonic development, tissue regeneration, and wound healing. One assay commonly used to study cell migration is the scratch assay creating a cell-free gap, or ‘scratch’, on a confluent cell monolayer. Whilst this method is popular, the inherent variability in how scratches are produced and physical damage to cells make it difficult to obtain quantitative and statistically valid measurements of cell migration. Furthermore it has been demonstrated that the ‘scratch’ will damage any underlying extracellular matrix (ECM). The Oris™ Cell Migration Assay has been shown, in comparative testing (1,2), to offer a superior alternative to the scratch assay, permitting formation of precisely placed and homogeneously sized cell-free areas into which migration can occur without releasing factors from wounded or dead cells. During cell seeding, Oris™ Assays use silicon stoppers or a non-toxic biocompatible gel (BCG) to form a centrally located and temporary cell-free zone on cell culture surfaces in a 96 or 384-wells format. After removal of the stoppers or dissolution of the BCG, the migration of cells into the detection zone can be observed either in real time or after further experimental processing. This breakthrough product enables researchers
to capture and quantify cell migration data while substantially improving assay efficiency and dramatically reducing assay costs. Measurement of cell migration enables researchers to better understand the impact of candidate therapeutic compounds on biological pathways and cellular behavior. The Oris™ cell migration platform offers a robust and statistically validated assay capable of supporting quantitative compound evaluation using a single determination per data point. Available from AMSBIO - ORIS™ Assay kits support quantitative analysis of compound structure-activity relationships and provide reliable and reproducible measures of compound potency either in medium or high throughput formats.
Circle no. 374
Rapid-Response Technical Support
Bibby Scientific supports all Jenway, Stuart and Techne equipment with comprehensive technical advice from a specialist team based on the manufacturing site.
Whether users require technical advice on a product or an application, a team of trained scientists with many years’ laboratory experience in research and industrial environments are on hand. Immediate advice is available by email or phone, and the aim is to respond to all enquiries within one working day.
With expertise in chemistry, biochemistry and molecular biology, the Jenway, Stuart and Techne Technical Support team is capable of responding to a wide range of enquiries. Access to a fully equipped on-site laboratory enables more complex problems and queries to be investigated. Close contact between the technical support experts, design engineers and service staff facilitates rapid information transfer, ensuring that customers receive high quality and comprehensive support.
Support topics range from advice on the best product for a specific application, to care and maintenance of equipment such as pH electrodes; from help with programming a thermal cycler, to analysis and troubleshooting of customers’ data. Information relating to spare parts and accessories, manuals for older pieces of equipment, and application notes is also provided to help users get the best performance from their Jenway, Stuart and Techne products.
Circle no. 375
For a better way to get your message across...
Contact:
sales@intlabmate.com Circle no. 376
LAB ASIA - MARCH/APRIL 2011 - LABORATORY PRODUCTS
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