8 True Blue Performance
Restek have introduced a range of GC injection liners that offer: increased accuracy and reproducibility with state-of-the-art deactivation: achieve lower detection limits for a wide range of active compounds; and use wool without risking the loss of sensitive analytes.
When faced with complex choices, simple solutions stand out. Sky™ inlet liners from Restek use a comprehensive, state-of-the-art deactivation and are the only blue liners on the market - making them an easy to recognise solution to common inlet problems.
The innovative deactivation used for Sky™ liners results in exceptional inertness for a wide range of analytes. By reducing active sites and enhancing analyte transfer to the column, these liners increase accuracy and precision, allowing lower detection limits for many active compounds. In addition to improved data quality, you’ll benefit from fewer liner changes and less downtime for maintenance.
Selecting the right liner for your application can be a daunting task. Sky™ inlet liners make the choice simple; the comprehensive deactivation, distinctive colour, and availability in popular configurations mean Sky™ liners are the best choice for optimising chromatographic performance. Regardless of your application, Sky™ liners provide reliable inertness and assured performance, day-after-day and analysis-after-analysis.
Circle no. 322
CHROMATOGRAPHY
SEC-LS Dual Integrated Detector Dedicated to Proteins
Malvern Instruments has launched the Zetasizer µV with OmniFACE and OmniSEC, a new SEC-LS (size exclusion chromatography - light scattering) detector package dedicated to protein analysis. The proven Zetasizer µV offers dual DLS (dynamic light scattering) and SLS (static light scattering) capabilities. It can be used as a stand-alone detector, analysing samples in a cuvette, and now as a fully featured modular chromatography detector. For protein applications, this makes the Zetasizer µV an ideal tool. Applications in stand-alone batch mode include the study of molecular size and formulation stability, and in SEC detector mode the measurement of molecular weight, determination of oligomeric state and detection and quantification of oligomers and aggregates. The absolute nature of the measurement means that no column calibration is required.
The Zetasizer µV is available as a complete chromatography system or can be added to existing SEC modules. Malvern’s OmniFACE provides the necessary connection that enables the Zetasizer µV to be linked into any third party SEC system and detectors, so that output signals can be used by the OmniSEC software for results calculation. Connecting the Zetasizer µV is simplicity itself. Using standard chromatography tubing it can be added in series with other detectors such as UV and RI. The laser stability and optical setup of the Zetasizer µV ensure excellent baseline stability and signal- to-noise for maximum sensitivity, while the 8µL flow cell minimises band broadening.
Circle no. 323
Safety First With HPTLC
Omicron Research Ltd bring customers the recently published Camag Bibliography Service (CBS) 105, focussing on food and water analysis using high performance thin layer chromatography. With food safety laws becoming more and more stringent, food analysis is increasingly important to many UK and international companies. One of the world’s most important food crops, the potato, is the subject of one study, using HPTLC for determination of alkaloids. At certain intensities alkaloids can render the humble potato harmful to human health. Using Camag’s Automatic Sampler ATS4, sensitive and selective analysis provided detailed and effective detection and quantification of alkaloid levels.
In contrast, the composition of PVC food wrap is also the subject of interrogation. Two methods of analysis were used in this study – mass spectrometry and biological activity. For rapid screening of additives, direct extraction of PVC plastic wrap using Camag’s TLC-MS Interface gave immediate top level results. For more comprehensive analysis, however, a TLC Chromatogram Immersion Device was used to dip plates in Vibrio Fischeri bacteria and documented with Camag’s Bioluminizer. Pollutants in water were also under the spotlight. Using automated multiple development and online analysis, drinking and raw water sources were monitored and analysed for contaminants and unknown substances.
Circle no. 324
Unique C18 Bonded HPLC Column
ACEâ C18-PFP is a unique C18 bonded HPLC column from Advanced Chromatography Technologies, which has been developed to provide alternate selectivity to all existing C18 bonded phases. The phase combines the hydrophobic characteristics of a C18 phase with the alternate selectivity offered by a pentafluorophenyl (PFP) phase – enabling the benefits of multiple interactions to be fully exploited. The phase also exhibits ultra low bleed to ensure LC/MS compatibility and demonstrates excellent temperature and pH stability. At launch, materials are available in 3µm, 5µm and 10µm particle sizes and a full range of column dimensions from high throughput through to preparative scale.
Circle no. 325 Circle no. 326
LAB ASIA - MARCH/APRIL 2011 - CHROMATOGRAPHY
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