Chromatography 19


Advantages of Combining Flow and Pressure Control


In a number of applications, both pressure and flow control play an important role in achieving system performance. One typical example of such an application is split/ splitless injectors in gas chromatography (GC).


In gas chromatography, the choice between


maintaining constant flow and maintaining constant pressure depends on a multitude of factors such as analytic type, sampling method and detector type. When maintaining pressure constant, flow rate can decline with increasing column temperature. This can lead to incompatibility issues with certain detector types, e.g. mass spectrometers and thermal conductivity detectors. When maintaining flow constant, higher pressures may need to be used, and this may fall out of the range of certain instruments.


Keeping the many advantages and disadvantages of flow and pressure control in mind, one possible solution is to combine both. Axetris mass flow meters and controllers can be easily combined with pressure sensors to build compact and fast-reacting flow+pressure controllers. Due to the quick reaction time achievable with the Axetris MEMS-based thermal mass flow technology, they lend themselves to easy integration as the controlling element in a pressure control loop. Axetris application experts help OEMs worldwide optimally combine flow and pressure control to achieve maximum performance.


42765pr@reply-direct.com SERVING ROYALTY. EXCEEDING EXPECTATIONS. EVERY MOMENT.


• Provider of top brand HPLC instrumentation products • Equivalent to corresponding OEM products • Serving customers for over 30 years • Reduce product repair expenses by 15% to 30% • Lifetime Warranty on manufacturing defects


www.sciencix.com 800.682.6480 sales@sciencix.com


7053ad@reply-direct.com


New Size Exclusion Columns for UHPLC


TSKgel UP-SW3000, a new 2 micron stationary phase, is the latest addition to the renowned TSKgel SW series of silica based gel filtration columns from Tosoh Bioscience. Packed into UHPLC column hardware it supports a smooth transfer of existing HPLC methods to UHPLC technology. TSKgel UP-SW3000 features the same surface modification and pore size as the well-established TSKgel G3000SWXL being the gold standard for QC of therapeutic antibodies in Biopharma for decades. Over 40 years


of experience in developing and producing SEC stationary phases are incorporated into the new series of UHPLC columns for the analysis of proteins in the range of 10 to 500 kDa.


Methods developed on conventional gel filtration columns can easily be transferred to UHPLC technology when using TSKgel UP-SW3000. The 30 cm column delivers increased resolution between mAb fragments, monomers, and aggregates. A 15 cm column is available to shorten analysis time while maintaining resolution. To achieve maximum resolution and fully exploit the potential of the columns the use of a UHPLC system is highly recommended. A so-called DC (direct connect) guard column can be attached directly to the analytical column to minimise extra column band broadening.


TSKgel SW columns stand out from other silica-based high performance size exclusion columns by virtue of their large pore volumes. They are based on highly porous, ultra-pure silica particles, the surface of which has been shielded from interacting with proteins by applying a proprietary surface chemistry. This ensures highest recoveries and accurate quantitation. The validated manufacturing and packing process delivers a reliable batch-to- batch reproducibility.


38895pr@reply-direct.com


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44  |  Page 45  |  Page 46  |  Page 47  |  Page 48  |  Page 49  |  Page 50  |  Page 51  |  Page 52  |  Page 53  |  Page 54  |  Page 55  |  Page 56  |  Page 57  |  Page 58  |  Page 59  |  Page 60  |  Page 61  |  Page 62  |  Page 63  |  Page 64  |  Page 65  |  Page 66  |  Page 67  |  Page 68  |  Page 69  |  Page 70  |  Page 71  |  Page 72  |  Page 73  |  Page 74  |  Page 75  |  Page 76  |  Page 77  |  Page 78  |  Page 79  |  Page 80  |  Page 81  |  Page 82  |  Page 83  |  Page 84  |  Page 85  |  Page 86  |  Page 87  |  Page 88  |  Page 89  |  Page 90  |  Page 91  |  Page 92  |  Page 93  |  Page 94  |  Page 95  |  Page 96  |  Page 97  |  Page 98  |  Page 99  |  Page 100  |  Page 101  |  Page 102  |  Page 103  |  Page 104  |  Page 105  |  Page 106  |  Page 107  |  Page 108  |  Page 109  |  Page 110  |  Page 111  |  Page 112  |  Page 113  |  Page 114  |  Page 115  |  Page 116  |  Page 117  |  Page 118  |  Page 119  |  Page 120  |  Page 121  |  Page 122  |  Page 123  |  Page 124  |  Page 125  |  Page 126  |  Page 127  |  Page 128  |  Page 129  |  Page 130  |  Page 131  |  Page 132  |  Page 133  |  Page 134  |  Page 135  |  Page 136  |  Page 137  |  Page 138  |  Page 139  |  Page 140  |  Page 141  |  Page 142  |  Page 143  |  Page 144  |  Page 145  |  Page 146  |  Page 147  |  Page 148  |  Page 149  |  Page 150  |  Page 151  |  Page 152  |  Page 153  |  Page 154  |  Page 155  |  Page 156  |  Page 157  |  Page 158  |  Page 159  |  Page 160  |  Page 161  |  Page 162  |  Page 163  |  Page 164  |  Page 165  |  Page 166  |  Page 167  |  Page 168  |  Page 169  |  Page 170  |  Page 171  |  Page 172