Fluorescence In Vivo Endomicroscopy
Figure 9: Ex vivo imaging of breast cancer mastectomy specimens using acriflavine, PARPi-FL and matched H&E histopathology. (A) Acriflavine staining of normal fat and connective tissue. (B) Absence of PARPi-FL staining of normal fat and connective tissue. (C) H&E staining of normal fat tissue. (D) Acriflavine staining of tumor. (E) PARPi-FL staining of tumor. (F) H&E staining of tumor. Images courtesy of Dr. Phillip Currie. Scale bars=100 μm.
gery greatly affect
Early detection of tumors and residual tumors aſter sur- tumor recurrence and patient survival.
PARP1 inhibitor-based detection of oral tumors has exhibited promising results in preclinical studies [59]. Recent reports also suggest its potential
in detecting oral, oropharyngeal,
esophageal, and cervical cancers in mice, swine, and human biopsies [60,61]. PARPi-FL has been used to identify tumor in ex vivo breast tissue samples in an unpublished study (Figure 9) and has potential as a breast cancer label in vivo. PARPi-FL was also used successfully in detecting oral cancer when applied as a mouthwash, and discriminated between fresh biopsied samples of the oral tumor and the surgical resection margin with more than 95% specificity. PARPi-FL, in combination with FIVE imaging, enables a combination of micro and macroscopic evaluation techniques for screen- ing, surveillance, biopsy guidance, fresh biopsy staining, in vivo diagnostic capability, and intraoperative margin delinea- tion [60]. Te development of specific fluorescent molecular markers, such as PARPi-FL, offers a range of diagnostic and intraoperative methods that were not previously possible with other imaging modalities. Zebrafish eyes have also been imaged to demonstrate
FIVE’s potential to obtain image z-stacks from live animals with good registration, enabling digital reconstruction of the tissue (Figure 10).
2021 May •
www.microscopy-today.com
Figure 10: Maximum intensity projection of a 187 µm depth color-coded z-stack of zebrafish eye imaged with 488 nm blue laser scanner. Zebrafish blood vessel endothelium is expressing GFP, and the eye is also stained with acriflavine.
23
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92
