Carmichael’s Concise Review Coming Events
2020 APT&M 2020 – Atom Probe Tomography & Microscopy
July 7–10, 2020 Oxford, England
https://aptm2020.web.ox.ac.uk
Microscopy & Microanalysis 2020
August 2–6, 2020 Milwaukee, WI
www.microscopy.org
emc2020: 17th European Microscopy Congress August 23–28, 2020 Copenhagen, Denmark
www.emc2020.eu
16th International Congress of Histochemistry and Cytochemistry (ICHC)
August 30–September 2, 2020 Prague, Czech Republic
http://ichc2020.com
Neuroscience 2020 October 24–28, 2020
Washington, DC
www.sfn.org/meetings/neuroscience-2020
2020 MRS Fall Meeting & Exhibit November 29–December 4, 2020 Boston, MA
www.mrs.org/fall2020
ASCB 2020 Annual Meeting
December 5–9, 2020 Philadelphia, PA
www.ascb.org/meetings-events/future-ascb-meetings
2021
Microscopy & Microanalysis 2021 August 1–5, 2021
Pittsburgh, PA
www.microscopy.org
2022
Microscopy & Microanalysis 2022 July 31–August 4, 2022
Portland, OR
www.microscopy.org
2023
Microscopy & Microanalysis 2023 July 24–28, 2023
Minneapolis, MN
www.microscopy.org
2024
Microscopy & Microanalysis 2024 July 28–August 1, 2024
Cleveland, OH
www.microscopy.org
Figure 1: Cryogenic super-resolution fluorescence microscopy of high-pressure frozen cells coupled with FIB-SEM enables multicolor 3D nanoscale visualization of proteins in the context of global ultrastructure. Clockwise from upper left: Volume-rendered cell with correlated orthoslice (inset) of mitochondria and endo- plasmic reticulum (ER) proteins; endolysosomal compartments of diverse morphology; heterochomatin sub- domains defined by protein reporters of transcriptional activity; adhesion proteins correlated to membrane roughness at contacting cerebellar granule neurons; and a peroxisome (pink) juxtaposed to an ER sheet (red) and mitochondrion (cyan). The size of the orthoslice on the left is 4×4 μm. The one on the right is 7.5×11.5 μm.
8 doi:10.1017/S1551929520000838 2020 May
Imaging Many Proteins Frozen In situ Stephen W. Carmichael* and Jeffrey L. Salisbury Mayo Clinic, Rochester, MN 55905
*
carmichael.stephen@
mayo.edu Te fundamental tenet of modern biological understanding is the relation-
ship between structure and function. Tat is, what something does is directly related to its shape, what it is made of, and the arrangement of its parts. Tis is true at every size scale from the organism, to the organ system, the tissue, the cell, the organelle, the macromolecular complex, and finally at the molecular level. Recent advances in microscopic imaging have provided game-changing progress that is important for the understanding of biological mechanisms. A large group led by Eric Betzig and Harald Hess, and including David Hoffman and Gleb Shtengel, recently used a correlative microscopy technique that combines three- dimensional (3D) fluorescence super-resolution (SR) microscopy and high-reso- lution volume reconstruction by block-face scanning electron microscopy (SEM).
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