Volume 28 Number 3 2020 May


National Cryo-EM Facility


12 The National Cryo-EM Facility at Frederick National Laboratory: Operational Procedures, Methods, and Outputs Ulrich Baxa, Thomas J. Edwards, Matt Hutchison, Adam D. Wier, Joseph Finney, Helen Wang, and Sriram Subramaniam


Contents Light Microscopy


18 ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations Taewoo Kim, Eugene Serabyn, Maximilian Schadegg, Kurt Liewer, Nathan Oborny, J. Kent Wallace, Stephanie Rider, Manuel Bedrossian, Christian Lindensmith, and Jay Nadeau


Spectroscopy


26 Optical Photothermal Infrared Microspectroscopy with Simultaneous Raman – A New Non-Contact Failure Analysis Technique for Identification of <10 µm Organic Contamination in the Hard Drive and other Electronics


Industries Mustafa Kansiz, Craig Prater, Eoghan Dillon, Michael Lo, Jay Anderson, Curtis Marcott, Abel Demissie, Yanling Chen, and Gary Kunkel


38 AFM-in-SEM as a Tool for Comprehensive Sample Surface Analysis Veronika Novotna, Josef Horak, Martin Konecny, Veronika Hegrova, Ondrej Novotny, Zdenek Novacek, and Jan Neuman


Scanned Probe Microscopy About the Cover


48 Electrical and Mechanical Characterization of Li Ion Battery Electrode using PinPoint™ SSRM John Paul Pineda, Cathy Lee, Byong Kim, and Keibock Lee


Microscopy 101


54 Recipes for Consistent Selected Area Electron Diffraction Results: Part 2: Recording the SAED Pattern Scott D. Walck


Pioneers of Microscopy


60 Nestor Zaluzec: Next Question, Please Cameron Varano


Highlights from Microscopy and Microanalysis


A Titan Krios microscope at the National Cryo-EM Facility and selected results. 2D classes produced on the fly during data collection (upper right), and two recent cryo-EM structures obtained from data collected at the facility (5-HT3A


serotonin receptor and Dot1L


nucleosome complex) are highlighted. For details, see the article by Baxa et al.


66 Determination of Mass Attenuation Coefficients of Th, U, Np, and Pu for Oxygen Kα X-Rays Using an Electron Microprobe


66 Confocal Analysis of Distribution and Persistence of Sindbis Virus (TaV-GFP) Infection in Midguts of Aedes aegypti Mosquitoes


67 Introducing and Controlling Water Vapor in Closed-Cell In Situ Electron Microscopy Gas Reactions


Departments


7 Editorial 8 Carmichael’s Concise Review


62 Industry News 64 Product News 68 Crossword Puzzle


70 NetNotes 78 Calendar of Meetings 81 Dear Abbe 82 Index of Advertisers


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