Microscopy 101
In Part 3 of this series, soſtware used to analyze SAED patterns will be discussed.
Acknowledgements Te research reported in this document was performed
in connection with contract/instrument W911QX-16-D-0014 with the U.S. Army Research Laboratory. (As of January 31, 2019, the organization is now part of the U.S. Army Combat Capabilities Development Command [formerly RDECOM] and is now called CCDC Army Research Laboratory.) Te views and conclusions contained in this document are
those of the author and should not be interpreted as presenting the official policies or position, either expressed or implied, of the CCDC Army Research Laboratory or the U.S. Government unless so designated by other authorized documents. Citation of manufacturer’s or trade names does not constitute an official
endorsement or approval of the use thereof. Te U.S. Government is authorized to reproduce and distribute reprints for Govern- ment purposes notwithstanding any copyright notation hereon.
References [1] Copy All Tags to Image.s, DRG Mitchell, Dave Mitchell’s DigitalMicrograph™ scripting website, http://www.
dmscripting.com.
[2] DRG Mitchell, Microsc Res Tech 71 (2008) 588–93. [3] DiffTools, Dave Mitchell’s DigitalMicrograph™ scripting website,
http://www.dmscripting.com.
[4] Invert Image Contrast.s, Dave Mitchell, Dave Mitch- ell’s DigitalMicrograph™ scripting website, http://www.
dmscripting.com.
Appendix A DM Script: SimpleImageSum.s // SimpleImageSum.s // Tis short script adds the intensities from one image to another image preserving the image tags. image img0, img1 gettwoimageswithprompt(“0=Image0 With Beam Block, 1=Image1 Without Beam Block”,\ “Add Two Images together”, img0, img1) img0=img0+img1
Appendix B DM Script: ReplaceCenter.s // ReplaceCenter.s // Tis is an alternative short script to replace the center of the image without beam block // with the center of the image with the beam block. image img0, img1 number cntrX=1995 // X position of center crossing lines number cntrY=1337 // Y position of center crossing lines number W=25 // 2W is size of square box ROI gettwoimageswithprompt(“0=Image0 With Beam Block, 1=Image1 Without Beam Block”,\ “Add Two Images together”, img0, img1) img0[cntrY-W, cntrX-W, cntrY+W, cntrX+W]=img1[cntrY-W, cntrX-W, cntrY+W, cntrX+W]
Appendix C DM Script: SimpleAutoDiffToolsTranspose.s //SimpleAutoDiffToolsTranspose.s //Arrange SAED Images: //Front Image - short exp without beam block //Next image - long exp with beam block.
image img000 number SX, SY, ROIdim GetFrontImage(img000) GetSize(img000, SX, SY) ROIdim=160 //ROI box is 160x160 pixels SetSelection(img000,(SY-ROIdim)/2, (SX-ROIdim)/2, (SY+ROIdim)/2, (SX+ROIdim)/2) ChooseMenuItem(“DiffTools”,“”,“Locate (000) Spot”) ChooseMenuItem(“DiffTools”,“”,“Transpose (000) Centre”)
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