Life Science Applications Blood Alcohols I (Static Headspace/Split)


Column: DB-ALC1 125-9134 30 m x 0.53 mm, 3.00 µm


Carrier: Helium at 80 cm/sec, measured at 40°C


Oven: 40°C Isothermal Sampler: Headspace Injection: Split, 250°C Split ratio 1:10


Detector: FID, 300°C Nitrogen makeup gas at 23 mL/min


1 Suggested Supplies


Septum: Advanced Green, 5183-4759 Liner: Direct, 1.5 mm ID, 18740-80200 Seal: Gold plated seal, 18740-20885


0 0.5 1.0 2 5 6 3


4


1. Methanol 2. Acetaldehyde 3. Ethanol 4. Isopropanol 5. Acetone 6. 1-Propanol


1.5 Time (min)


2.0


2.5


3.0 GCLS023


Blood Alcohols II (Static Headspace/Split)


Column: DB-ALC1 125-9134 30 m x 0.53 mm, 3.00 µm


Carrier: Helium at 80 cm/sec, measured at 40°C


Oven: 40°C Isothermal Sampler: Headspace Oven: 70°C Loop: 80°C Transfer Line: 90°C Vial Equil. Time: 10 min Pressurization Time: 0.20 min Loop Fill Time: 0.20 min Loop Equil. Time: 0.05 min Inject Time: 0.1 - 0.2 min Sample Loop Size: 1.0 mL


Injection: Split, 250°C Split ratio 1:10


Detector: FID, 300°C Nitrogen makeup gas at 23 mL/min


Sample: 0.1% Ethanol, 0.001% Others


3 4 Suggested Supplies


Septum: Advanced Green, 5183-4759 Liner: Direct, 1.5 mm ID, 18740-80200 Seal: Gold plated seal, 18740-20885


1 2 6


5


1. Methanol 2. Acetaldehyde 3. Ethanol 4. Isopropanol 5. Acetone 6. 1-Propanol


0.5


1.0


1.5 Time (min)


2.0


2.5


3.0 GCLS024


Order online at www.agilent.com/chem/store


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