FAMEs
Column: DB-23 122-2362 60 m x 0.25 mm, 0.25 µm
Chromatogram provided courtesy of SteveWatkins and Jeremy Ching, FAME Analytics,
http://www.fameanalytics.com
Carrier: Hydrogen at 43 cm/sec Constant pressure mode
6 9 8 5 3 4 1 10 11 19 13 15 16 17
20 18
2 22 23 24 2526 27 28 7 12
Baseline Resolution of C16 and C18 cis-, trans- isomers
14 21
Oven: 130°C for 1.0 min 130-170°C at 6.5°C/min 170-215°C at 2.75°C/min 215°C for 12 min 215-230°C at 40°C/min 230°C for 3 min
Injection: Split, 270°C Split ratio 50:1
Detector: FID, 280°C 29 303132 33 34 35 36 37 383940 41
2 468 10 12 14 16 18 20 22 24 26 28 Time (min)
GCFF027 Suggested Supplies
Septum: Advanced Green, 5183-4759 Liner: Split, single taper, low pressure drop, glass wool, 5183-4647 Seal: Gold plated seal, 18740-20885 Syringe: 5 µL tapered, FN 23-26s/42/HP, 5181-1273
1. C6:0 2. C7:0 3. C8:0 4. C9:0 5. C10:0 6. C11:0 7. C12:0 8. BHT 9. C13:0 10. C14:0 11. C14:1n5 12. C15:0 13. C16:0 14. C16:1n7(trans) 15. C16:1n7(cis) 16. C17:0 17. C17:1 18. C18:0 19. C18:1n9(trans) 20. C18:1n9(cis) 21. C18:1n7 22. C18:2n6 23. C18:3n6 24. C18:3n3 25. C18:2(d9,11) 26. C18:2(d10,12) 27. C20:0 28. C20:1n9 29. C20:2n6 30. C20:3n6 31. C20:4n6 32. C20:3n3 33. C20:5n3 34. C22:0 35. C22:1n9 36. C22:2n6 37. C22:4n6 38. C22:5n3 39. C24:0 40. C22:6n3 41. C24:1n9
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