Life Science Applications Benzodiazepines


Column: DB-5ms Ultra Inert 122-5532UI 30 m x 0.25 mm, 0.25 µm


Carrier: Oven:


Hydrogen, 53 cm/sec, constant flow 1.6 for 11 min 1.6 to 2.4 at 60 mL/min2 hold 2 min 2.4 to 5.0 at 50 mL/min2 hold 9 min


9 0 pA


Injection: Pulsed Splitless, 280°C 20 psi pulse pressure for 0.38 min 50 mL/min purge at 0.40 min Direct Connect liner G1544-80730


170°C for 3.2 min 170-250°C at 24.7°C/min, hold 5.3 min 250-280°C at 18.6°C/min, hold 4.0 min 280-325°C at 50.0°C/min, hold 4 min


Detector: FID, 350°C Sample: 1 µL of 5-10 ppm


20 30 40 50 60 70 80


10 78 9 10 11 Time (min)


Analysis of benzodiazepines and other drugs is particularly challenging because of their high level of activity. For this reason, all aspects of the sample path – particularly the GC Column – must be as inert as possible.


BENZODIAZ 12 13 1 2


1. Medazepam 2. Halazepam 3. Oxazepam 4. Lorazepam 5. Diazepam 6. Desalkyl Aurazepam 7. Nordazepam 8. Clonazam 9. Oxazopam


7 5 8 16 19 17 11 12 6 9 3 4 10 14 18 21 20


10. Temazepam 11. Flunitrazepam 12. Bromazepam 13. Prazepam 14. Lormetazepam


15 13


15. Nitrazepam 16. Chlordiazepoxide 17. Clonazepam 18. Demoxepam 19. Estazolam 20. Alprazolam 21. Triazolam


Ramped Flow 14


15


16


Amphetamines and Precursors - TMS Derivatives


Column: DB-5 121-5023 20 m x 0.18 mm, 0.40 µm


Carrier: Helium at 39 cm/sec, measured at 100°C Oven: 100-240°C at 10°/min Injection: Split, 250°C Split ratio 1:100


Detector: FID, 300°C Nitrogen makeup gas at 30 mL/min


Sample: 1 µL of 2 µg/µL each in pyridine


1. Phenylacetone 2. Dimethylamphetamine 3. Amphetamine 4. Phentermine 5. Methamphetamine 6. Methyl ephedrine 7. Nicotinamine 8. Ephedrine


9. Phenacetin 10. 3,4-Methylenedioxyamphetamine (MDA) 11. 3,4-Methylenedioxymethylamphetamine 12. 4-Methyl-2,5-dimethoxyamphetamine (STP) 13. Phenyl ephedrine 14. 3,4-Methylenedioxyethylamphetamine (MDE; Eve)


15. Caffeine 16. Benzphetamine


14 min GCLS004 1 3 4 67 8 9 5 10 2 15 12 11 14 16 13 Suggested Supplies


Septum: Advanced Green, 5183-4759 Liner: General purpose split/splitless liner, taper, glass wool, 5183-4711


Seal: Gold plated seal, 18740-20885 Syringe: 10 µL tapered, FN 23-26s/42/HP, 5181-1267


Order online at www.agilent.com/chem/store


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