Food, Flavor, and Fragrance Applications Spearmint Oil


Using hydrogen as a carrier gas in conjunction with the high efficiency column resulted in an overall speed gain of 61% compared to the the original method. In addition, the resolution was well maintained throughout the method translation process.


Column A: DB-1 122-1032 30 m x 0.25 mm, 0.25 µm


Column B: DB-1 121-1022 20 m x 0.18 mm, 0.18 µm


OriginalMethod with a DB-1, 30 m x 0.25 mm, 0.25 µm column and Helium carrier


A A 9 21


1. a-Pinene 2. Sabinene


5. Myrcene 5 25 8 23 4 1 23 8 10 67 10 12 13


11 12


14 15 14 16 17 16


18 19


20 18 Time (min)


FasterMethod with a high efficiency DB-1, 20 m x 0.18 mm, 0.18 µm column and Hydrogen carrier


B 9 21 20 22 27 29 24 26 22 28 24 26 30 31 28


3. b-Pinene 4. 3-Octanol


6. a-Terpinene 7. ρ-Cymene 8. 1,8-Cineol 9. Limonene 10. cis-Ocimene 11. trans-Ocimene 12. g-Terpinene 13. trans-Sabinene hydrate 14. Terpinolene 15. Linalool 16. 3-Octyl acetate 17. Isomenthone 18. Terpinen-4-ol 19. Dihydrocarvone 20. trans-Carveol 21. l-Carvone 22. trans-Dihydrocarveol acetate 23. cis-Carvyl acetate 24. cis-Jasmone 25. b-Bourbonene 26. a-Bourbonene 27. b-Caryophylene 28. a-Copaene 29. trans-b-Farnesene 30. Germacrene-d 31. Viridiflorol


8 5 10 11 13 1 2 34 67 12 14 15 16 17 19 18 20 22 23 24 27 30 26 28 29 31


3579 11 Time (min)


SPEARMINT S 25


Carrier: Oven:


A: Helium 25 cm/sec measured at 40°C B: Hydrogen 47 cm/sec measured at 40°C


Injection: 250°C, Split 40:1, 1 µL injection


A: 40°C hold 1 min, 5°C/min to 290°C B: 40°C hold 0.38 min, 13°C/min to 290°C hold 13.09 min


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