Column Installation and Troubleshooting
5. Make a clean break. Grasp the column between the thumb and forefinger as close to the score point as possible. Gently pull and bend the column. The column should part easily. If the column does not break easily, do not force it. Score the column again in a different place (farther from the end than before) and try again for a clean break.
6. Use a magnifying loupe to inspect the cut. Make sure the cut is square across the tubing with no polyimide or "glass" fragments at the end of the tube.
7. Install the column in the inlet. Check the GC manufacturer’s instrument manual for the correct insertion distance in the injection port type being used. Slide the column nut and ferrule to the proper distance and then mark the correct distance on the column with typewriter correction fluid just behind the column nut. Allow the fluid to dry. Insert the column into the injector. Finger tighten the column nut until it starts to grab the column, and then tighten the nut and additional 1/4 to 1/2 turn, so that the column cannot be pulled from the fitting when gentle pressure is applied. Verify that the correct column insertion distance has been maintained by looking at the typewriter correction fluid mark.
8. Turn on the carrier gas and establish the proper flow rate. Set head pressure, split flow, and septum purge flow to appropriate levels. See Table 7 for nominal head pressures. If fusing a split/splitless inlet, check that the purge (split) valve is "on" (open).
9. Confirm carrier gas flow through the column. Immerse the end of the column in a vial of solvent and check for bubbles.
10. Install the column into the detector. Check the instrument manufacturer’s manual for the proper insertion distance.
11. Check for leaks. This is very important. Do not heat the column without thoroughly checking for leaks.
12. Establish proper injector and detector temperatures. 13. Establish proper makeup and detector gas flows. Ignite or turn "on" the detector.
14. Purge the column for a minimum of 10 minutes at ambient temperature. Add the appropriate additional purge time following inlet or trap maintenance.
15. Inject non-retained substance to check for proper injector installation. Examples: butane or methane (FID), headspace vapors from Acetonitrile (NPD), headspace vapors from methylene chloride (ECD), air (TCD), argon (mass spectrometer). Proper installation is indicated by a symmetrical non-retained peak. If tailing is observed, reinstall the column into the inlet.
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