MALDI-MS imaging for the study of tissue pharmacodynamics & toxicodynamics Perspective
A
B
C E
D
Figure 2. Combinations of techniques can provide validation of pharmaco/toxicodynamic data. (A) Regression vector plots used in partial least squares discriminant analysis, (B) both labeled and label-free quantitative MS, (C) alternative imaging modalities (e.g., MRI), (D) immunohistological staining and (E) MALDI-MS imaging with ion mobility technology are regularly used in dose response relationship studies.
obtained against current practice, a high-throughput capability as previously discussed, demonstrable cost effectiveness as an analytical tool and, of great impor- tance, the establishment of a good communication network between the mass spectrometrist, clinician and patient. Instantaneous mapping, localization and coregis-
tration of species still remain a highly desirable fac- tor, and is one of the main triumphs of the use of MALDI-MSI in tumor dose response relationship studies. If MALDI-MSI is used in combination with
future science group
complementary conventional MS techniques (Figure 2) and in vivo imaging modalities (e.g., MRI), then there is the potential for such studies to provide a facile route to biomarker discovery and reveal molecular targets for future drug design [34,35].
PD/TD responses in lung studied by MALDI-MSI While there appear to be no reports of PD/TD stud- ies that employ MALDI-MSI being carried out on the lung, a number of drug distribution studies have been
www.future-science.com 95
Reg vector for Y 2
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