Editorial
Microsampling – a look back at 2015
Keywords: blood • bioanalysis • DBS • microsampling • plasma • quantitation
Since the very first issue of Bioanalysis in 2009, articles covering various aspects of microsampling for the determination of cir- culating analyte concentrations have been regular visitors to the pages of the journal [1]. Through this journal, bioanalysts have been able to share the developments in the tech- nology, understand the benefits and issues associated with their use, learn of improve- ments in workflows and data quality, and demonstrate their practical utilization in authentic study and population scenarios. This heritage has continued into 2015, where during the year over 40 manuscripts have continued to engage the bioanalytical com- munity with this topical subject, including a special issue dedicated to it. It is also impor- tant to note that at the time of writing, six of the top ten cited articles in the journal have microsampling as the major subject focus. Microsampling truly is one of the mainstays of the Bioanalysis journal.
Novel collection technologies In 2015, readers of Bioanalysis have wit- nessed the introduction of a number of novel microsampling approaches whose aim is to improve the quality of the sample collected and the data derived from it. One particu- lar focus has been overcoming the effect of blood hematocrit on blood volume for dried blood sampling. A number of publications have explored the
novel Mitra volumetric absorptive microsam- pler (VAMS), for the collection of dried blood samples. These manuscripts have demon- strated the simplicity of the approach, how the
10.4155/bio.16.14 © 2016 Future Science Ltd
effect of blood hematocrit on sample volume observed with dried blood spots (DBS) can be minimized and how the effect of HCT on recovery can be decreased. Further, the utiliza- tion of the device for routine clinical and pre- clinical toxicokinetic (TK) sampling and its use for drug discovery pharmacokinetic (PK) studies in rodents has been established [2–7]. Another novel approach for the collection of
DBS with a fixed blood volume was reported by Lenk and colleagues [8]. The prototype was demonstrated to collect approximately 6 µl with excellent accuracy and is put forwards as a potential means of overcoming the effect of hematocrit on DBS blood volume sampled, while maintaining the established advantages of the DBS sampling approach. Mengerink and colleagues published a
manuscript outlining a novel hydrophilic- coated woven polyester substrate for DBS sampling that showed good homogeneity and a reduced effect of hematocrit on spot size compared with conventional cellulose-based substrates [9]. For situations where a dried plasma micro-
sample would be beneficial, Sturm and co-workers introduced a membrane-based device that produces a dried plasma sample from whole blood, without the need for cen- trifugation and, hence, offering a simplified process [10]. An approach that combines microsampling
with a simple low volume analytical approach was reported by Boonyasit and Laiwattanapa- isal. Their microfluidic device is capable of acquiring
albumin-corrected fructosamine values from a 45 µl aliquot of whole blood that Bioanalysis (2016) ISSN 1757-6180
Neil Spooner Spooner Bioanalytical Solutions, Hertford, Hertfordshire, UK and, Department of Pharmacy, Pharmacology and Postgraduate Medicine, University of Hertfordshire, College Lane, Hatfield, AL10 9AB, UK
Neil@spoonerbioanalytical.co.uk
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