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Web-Based Interactive Measurements


for the segmented mask selected as the input and viewing area shown in Figure 5 . T e resulting information can be used to assess a spatial density of particles of a particular area in pixels {[red, 1352], [green, 1338], [blue,1161], [yellow, 1035], [pink, 825]} or in nm 2 by multiplying the pixel area by 6534 nm 2 (the pixel dimensions 48.5 nm × 48.5 nm divided by the zoom level 0.6 × 0.6 equals to 6,534 nm 2 ). If the objective is to measure the length of particles (or any other objects of interest) then distance


measurements can be obtained by mouse clicks aſt er checking


the check box “Active measurement.” Figure 8 shows distance results for the elongated nanofi bers with color-coded points and their corresponding measurements in SI units {12, 10, 8, 8, 10, 6, 5, 5, 6, 7} [μ m] (average =7.7 μ m, standard deviation = 2.36 μ m).


Discussion


A combination of assembly computations yielded a large FOV and proved to be robust to varying SNRs, feature types, and amount of overlap in each input collection of thousands


Figure 7 : When the button “Run Analysis” is pressed, an image is binarized into black and white pixels by thresholding (that is, white pixel if above and black pixel if below intensity threshold), all neighboring pixels of the same binarized pixel are connected into regions (connectivity analysis), and a unique number/color is assigned to each connected region. The right panel presents color-coded connected regions sorted by area and total count of regions viewed in a browser window (in this case, the viewing area is shown in Figure 5 and consists of rod-like particles of interest and extraneous round polymer fragments).


Figure 8 : Distance measurements are obtained by mouse clicks on end points of nanofi bers. The measurements are recorded in the left pane and can be deleted or downloaded (clicked point coordinates and distance).


24 www.microscopy-today.com • 2017 January


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