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Web-Based Interactive Measurements


Table 1 : A list of data and computational jobs accessible via web user interfaces.


Data


Tile collections Stitching vectors Image pyramids Visualizations


Computational Jobs Flat field correction Filtering Stitching


Segmentation Pyramid building Intensity scaling Tessellation


Figure 2 : An illustration of multi-resolution pyramid representation of images of dye particles on printing paper. The vertical axis corresponds to the level of details. The red line in each horizontal plane denotes spatial partitioning into overlapping pyramid tiles.


of 40 × 59 image tiles. Each FOV image is 6.3 MB (6 MiB 1 , 3,072 × 2,048 pixels, 8 bits per pixel, 49 × 49 nanometer pixels). Another similar set of test images was acquired by imaging dye particles on printing paper with the same microscope. T e small FOVs have an overlap of 10% in horizontal and vertical 1https://en.wikipedia.org/wiki/Mebibyte


directions, and they form a grid of 20 × 20 raw image tiles of the same pixel size. In both data sets, the goal is to characterize size, shape, and spatial distribution of these small particles in a spatially large specimen. Main methods . We designed a web-based (client-server) system to handle the following tasks: (1) uploading collections of small FOVs to a web application with access to computer cloud resources, (2) confi guring the computations to assemble a large FOV , (3) viewing and exploring the large FOV via an interface for spatial pan-zoom and time/spectral-sweep, and (4) executing interactive and collaborative measurements over the large FOV


Figure 3 : The interface to “Data” and computational “Jobs” functions at the top of the web application is via drop-down menus. The accordion menu on the left lower side contains fi lters, connectivity analysis to obtain counts of objects, and distance measurements that are linked to the viewing area on the right side. Any change of fi lter parameters will be refl ected in the image display while different areas at multiple resolutions are explored using pan and zoom, and distances are measured by activating the measurement tool and using mouse clicks.


20 www.microscopy-today.com • 2017 January


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