78 MICROSCOPY & IMAGING
CELLULAR IMAGING ACQUISITION and ANALYSIS SYSTEM
powerful software component for accessing and creating Raman spectral databases. It can search existing libraries of Raman spectra to identify sample components and also allows users to create their own catalogue of acquired spectra to assist in their research. TrueMatch is available as a fully
W integrated option with the WITec Project
ITec, manufacturer of 3D Raman imaging systems, is now offering TrueMatch, a
Five software environment and databases can be brought directly into use in post-processing. Multi-spectral searches enable the user to identify several spectra simultaneously, and return hit rates that show the probability of a match for each individual spectrum. This greatly accelerates the process of characterisation and is a perfect complement to WITec’s standard data analysis features. Demix search uses databases to
describe acquired spectra in terms of pure substances, which can then be physically located in an image. Seeing the distribution of pure chemical components matched to sample features gives the researcher a precise visualisation. Generated reports can be stored for further use in subsequent measurements, amplifying the effectiveness of the system as a whole and turning
meaningful results into a valuable resource. In addition to the proprietary WITec
Raman database capability included with TrueMatch, compatible versions of ST Japan’s wide range of databases are available. Extensive libraries of Raman spectra can be referenced while new ones are created, all from within WITec’s fully integrated software and hardware environment. “TrueMatch makes it easier for the user
to interpret their Raman images. It also greatly simplifies the process of searching through Raman spectral databases and creating new ones based on user-generated data compared to anything else out there. It’s the perfect complement to the standard analysis methods WITec implements in our Suite Five software environment,” says Olaf Hollricher, managing director and director of Development at WITec.
For more information visit
www.witec.de
NEXT-GENERATION MICROSCOPE
microscope that not only provides optical sectioning with optimal spatial resolution and signal to noise ratio for fixed or live specimen, but also delivers a high temporal resolution for 3D acquisition, addressing the needs for dynamic imaging of rapid biophysical processes.
A
lpha3 is a new generation of light sheet fluorescence
From in-vivo imaging to large cleared
samples, the new microscope delivers excellent image quality while keeping the necessary flexibility and modularity expected for cutting-edge scientific research instruments. The microscopy system architecture allow for a large choice of detection objectives, from low to high magnification and numerical aperture, including long working distance objectives with correction collars especially designed for being used with clearing solutions. Based on a light sheet technology, the Alpha3 system provides the sharpest optical sectioning (focus area) over the entire field of view in real time while suppressing “streaks” artifacts, producing optimum image quality for qualitative and quantitative imaging. In addition, by integrating an innovative fast remote focusing method, it allows for high-speed 3D acquisition at camera frame rate without using motorised means. This fully meets the requirements for 3D+time imaging of
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morphogenetic events in living organisms or the observation of fast dynamic processes at sub cellular level and ensures no specimen perturbation that could introduce imaging artifacts compromising further analysis. Special attention has been given to the chamber design and its sample mounting accessories to facilitate sample holding and inspection. The chamber is well adapted for live small specimens while having the useful dimensions for large samples such as a whole mouse brain. Alpha3 typical applications include in
toto imaging of small animal models such as whole mouse embryos, morphogenesis and embryogenesis of C.elegans, Drosophila, Zebrafish, live imaging of cell cultures, functional imaging of neuronal activity, fluorescence imaging of marine organisms or plant developmental biology.
For more information visit
www.alphalightsheet.com
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