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36


Affordable, automated single cell RNA sequencing


Integra Biosciences and Parse Biosciences are pleased to announce a new partnership aimed at automating single cell RNA sequencing (scRNA-seq) using the ASSIST PLUS pipetting robot. This innovative protocol combines Parse Biosciences’ Evercode™ combinatorial barcoding technology with Integra’s advanced liquid handling systems, offering laboratories in both academic and industrial settings a streamlined and standardised approach to sequencing. The result is faster throughput and more consistent, reproducible outcomes.


scRNA-seq is pivotal for advancing research and developing therapies in fields such as developmental biology, neurology, diabetes, cardiovascular disease, and cancer. Parse Biosciences, located in Seattle, is renowned for its pioneering tools in scRNA-seq at scale. Their Evercode technology allows for the preparation and sequencing of up to one million cells or nuclei in parallel through a unique labelling process, eliminating the need for individual cell isolation.


In collaboration with Integra, Parse Biosciences has developed an automated scRNA-seq solution designed to overcome challenges associated with traditional manual workflows and costly specialised instruments. By integrating Evercode technology with the ASSIST PLUS pipetting robot, featuring the D-ONE single-channel pipetting module and the VIAFLO 8-channel electronic pipette, this new solution offers an easy-to-use, cost- effective workflow. It reduces hands-on time by 75% and manual pipetting steps by 85%, significantly easing the workload for lab staff.


Dr Charlie Roco, Co-Founder and CTO of Parse Biosciences, remarked: “Our successful collaboration with Integra has made automation and scRNA-seq more accessible to labs of all sizes and budgets. Automating the initial phase of our Evercode protocol on the ASSIST PLUS has accelerated workflows and minimised human error, facilitating more reliable high-quality scRNA-seq data. We have also developed additional workflows on the ASSIST PLUS compatible with Evercode TCR and Evercode BCR for T-cell and B-cell receptor profiling, and we anticipate further advancements in automating the rest of our scRNA-seq protocol on this platform soon.”


More information online: ilmt.co/PL/25ky 63031pr@reply-direct.com


Versatile titrator for simultaneous two-line titrations


The AUT-801 from DKK-TOA Corporation is a versatile titrator catering to a range of applications, including the capability for simultaneous two- line titration.


For simultaneous titration of two series, an optional burette can be added to the standard configuration, enabling concurrent performance of different titrations such as neutralisation and oxidation-reduction. This flexibility extends to applications such as simultaneous pH measurement and neutralisation titration. The system can be configured as a single system or as a dual-strain simultaneous titration system with additional components like the titration burette (ABT-8), stirrer (ST-8), electrode, electrode stand, and electrode holder.


The titrator supports a variety of titrations with its line-up of high-performance electrodes, including a pH glass electrode (Strong-pH) known for durability. Options such as ORP electrodes for redox titration, silver composite electrodes for salinity titration, electrodes for non-aqueous titration, and EC cells for electrical conductivity titration are available to accommodate various titrate ion methods.


Featuring 2 channels of pH/mV input as standard, the AUT-801 allows for the addition of optional titration units (light intensity, polarisation, electrical conductivity, potential difference) to the 3rd and 4th channels. The data memory function enables the storage of up to 600 data points in the main body for single-system use.


Moreover, the titrator is equipped with a validation support function, including a pH calibration history (up to 20 calibrations) and a burette capacity verification history creation function. AUT-801 stands as a comprehensive solution for precise and efficient titration processes across diverse applications.


More information online: ilmt.co/PL/zz9e 61764pr@reply-direct.com


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