27 Reagents, Reference Materials & Laboratory Chemicals


Tailored Taq polymerases for qPCR and NGS-based protein assays


Biotium, a leading provider of fluorescent reagents for life science research, has expanded its trusted Cheetah™ Taq DNA Polymerase line with two powerful new formulations: Cheetah™ FLEX HotStart Taq DNA Polymerase and Cheetah™ PEA-PLEX HotStart Taq DNA Polymerase. These innovative products are engineered to meet the needs of today’s high-performance molecular workflows, from fast- cycling qPCR to proximity extension assays (PEA) and NGS-based high-throughput PEA for protein analysis.


Both formulations build on Biotium’s proprietary chemically modified, antibody-free hot-start technology, designed to reduce non- specific amplification without the limitations of antibody-based systems. The result is faster activation, improved stability, and broader compatibility with modern PCR applications.


Cheetah™ FLEX HotStart Taq is optimised for speed and versatility. It features a rapid 2-minute activation at 95°C - up to 10 times faster than AmpliTaq Gold® or HotStartTaq®—without the risk of animal DNA contamination. This antibody-free approach not only improves long-term storage stability but also ensures reproducibility across fast-cycling PCR and qPCR-based PEA workflows. Once heat activated, Cheetah™ FLEX performs with the same fidelity and enzymatic properties as native Taq polymerase, making it suitable for both routine and advanced protocols.


For researchers working with high-throughput PEA and NGS-based workflows, Cheetah™ PEA-PLEX HotStart Taq offers a purpose-


built solution. Developed specifically for protein detection applications, it has been validated by third parties for use in NGS-based PEA systems and is also compatible with fast-cycling qPCR. Its formulation allows for seamless integration into demanding high-throughput assay environments.


Key benefits of both formulations include a unique chemical hot-start mechanism (no antibodies or activation lag), rapid 2-minute activation, enhanced contamination control due to the low-DNA enzyme preparation, and better stability than traditional chemically modified Taq enzymes. Cheetah™ PEA-PLEX is also available in ready-to-use kits or customisable bulk formats to suit the needs of commercial assay developers.


Both Cheetah™ FLEX and Cheetah™ PEA-PLEX are available now for purchase in standard and bulk quantities.


These latest additions join Biotium’s growing suite of advanced PCR tools, including the Forget-Me-Not™ qPCR Master Mixes and EvaGreen® Dye - formulated to deliver performance, reliability, and convenience for today’s molecular biologists. More information online: ilmt.co/PL/A3YB and ilmt.co/PL/vRpA


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Validated reagent kits for HCP quantifi cation


Gyros Protein Technologies AB, a leader in automated nanolitre-scale immunoassays and advanced peptide synthesis, has announced the release of two new host cell protein (HCP) quantification kits: the Gyrolab® HEK293 HCP Type SN Kit Reagents and Gyrolab HEK293 HCP Type CL Kit Reagents. These kits, developed in collaboration with BioGenes GmbH, are designed to support streamlined and sensitive HCP detection in biotherapeutics derived from HEK293 cell lines.


HCP removal is essential in biologics development to ensure product purity and safety. These impurities can provoke immune responses ranging from mild allergic reactions to severe complications, making their removal a regulatory and clinical priority. The new Gyrolab kits offer a robust solution for detecting HCPs with improved sensitivity and broad antigen coverage.


The kits are fully validated and ready to use on Gyros Protein Technologies’ automated Gyrolab immunoassay platform. By offering pre- optimised, plug-and-play reagent kits, the company enables faster, more reproducible HCP analysis with reduced sample and reagent consumption - improving both throughput and efficiency in analytical workflows.


While Gyrolab users have previously been able to use BioGenes’ antibodies with custom methods, the launch of these standardised kits simplifies implementation and enhances performance. The kits benefit from BioGenes’ proprietary 360-HCP antibody development strategy, which uses multiple host species, refined antigen preparation, and optimised purification to ensure exceptional antigen coverage and antibody quality.


More information online: ilmt.co/PL/Kwzw 64848pr@reply-direct.com


GMP-grade stem cell media simplifi es transition to clinical manufacturing


AMSBIO has introduced GMP-grade StemFit® for Differentiation, a chemically defined, animal component-free medium designed for consistent and efficient differentiation of human induced pluripotent stem (hiPS) and embryonic stem (hES) cells. Now compliant with Good Manufacturing Practices (GMP), this formulation supports seamless progression to clinical applications.


By eliminating animal-derived components, StemFit® for Differentiation minimises immunogenic risks while ensuring lot-to-lot consistency. Manufactured under strict GMP guidelines, it provides a reliable solution for the reproducible expansion and differentiation of stem cell-derived tissues in clinical settings.


Dr Satoshi Okamoto of Yokohama City University Graduate School of Medicine noted: “Using StemFit® Basic03 for iPSC culture alongside StemFit® for Differentiation, we established a stable, animal-origin-free protocol.”


By the end of 2025, all StemFit® formulations, including Basic03 and Basic04 CT, along with key proteins such as bFGF and Activin A, will be available exclusively as GMP-grade versions. This transition enables labs to move from research to clinical manufacturing without the need to retest materials or adjust protocols, reducing both complexity and costs.


To make GMP adoption accessible, AMSBIO has optimised manufacturing to minimise cost differences between research and GMP-grade products


Aisha Amari, a stem cell specialist at AMSBIO, commented: “With nearly 1,500 citations and growing use in cell and gene therapy, StemFit® has become a trusted solution for researchers and biotech companies worldwide.” Contact AMSBIO for further details about GMP compliant products and services. More information online: ilmt.co/PL/dYLV and ilmt.co/PL/y5mY


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