11 Sample Preparation & Processing
Figure 3: The results of testing on average change in weight over one month with three capping options.
Figure 4: Compression seal on external thread sample storage tube
Figure 3 shows the results of testing on average change in weight over one month with three capping options; traditional Internal O-Ring Cap, New Azenta External Cap and the New Azenta Internal Co-Mold Cap. Both the New External Cap and New Internal cap demonstrate excellent sealing, whilst the traditional Internal O-Ring Cap showed signifi cant evaporation after one month.
The Azenta External Thread Sample Tube was designed for a greatly increased burst pressure for enhanced sample integrity, it also has a range of other benefi ts including:
• Increased working volume allowing users to store more sample • Reduced tube height enabling a higher density of storage • Prevention of cross-threading and ‘jumping’ if the tube is over-torqued • Improved robustness of automated capping and decapping processes
These improvements are only possible with highly advanced manufacturing techniques, and therefore a rigorous quality control process. Details of the tolerances can be seen in Figure 4.
Summary
Due to the advanced manufacturing techniques used when Azenta tubes and caps are manufactured the results demonstrate the superiority of the New Internal Co-Mold Cap and New External Cap over the traditional Internal O-Ring Cap. This shows how two of the major hazards to compromising sample integrity; evaporation and cross contamination can be mitigated with the use of an effective capping method.
Reliable and robust capping can be assured through high quality manufacturing processes including a rigorous quality control process, simulating the expansion of aqueous samples during freezing and testing mechanical leaks.
The results all indicate that the new Azenta External Thread Cap provides an assured level of quality to maintain sample integrity whilst offering a range of other benefi ts including; higher density of storage, enabling automation, manual handling and increased working volume vs. internal thread tubes of the same size.
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www.labmate-online.com Innovative IR hotplate/stirrer for precision lab applications
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ilmt.co/PL/XOKk 61804pr@reply-direct.com Demonstration video shows automated peptide synthesis in action
A new demonstration video from Vapourtec showcases how its patented Variable Bed Flow Reactor (VBFR) is transforming solid phase peptide synthesis (SPPS) - offering fully automated workflows, sharper process control, and higher purity crude peptides.
The video walks viewers through an SPPS cycle, starting with the swelling of Tentagel S resin in DMF and continuing through Fmoc deprotection using 20% piperidine in DMF. A visible decrease in resin volume, tracked in real time by the reactor’s volume graph and a UV signal at 365 nm, marks this deprotection step with a clear Fmoc peak.
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