Mass Spectrometry & Spectroscopy 25 Environmental considerations
UV-Vis: • Temperature control: Maintain a stable temperature during measurements. Temperature affects absorbance.
• Solvent choice: Different solvents can alter absorption spectra. Choose a solvent compatible with your sample.
Fluorescence:
• Avoid photobleaching: Limit exposure to intense light to prevent fluorophore degradation.
• Shield from ambient light: Fluorescence is sensitive; even room lighting can affect measurements.
Conclusion
Individually and when combined, these molecular spectroscopy technologies enable scientists to advance our knowledge and understanding of biomolecules. Both UV-Vis and Fluorescence spectroscopy are simple, cost effective and powerful for quantification and characterisation and are well-established techniques. Researchers can use both techniques side by side and gain greater insight for biological research.
References
1: Agilent Technologies (n.d.). High-Throughput Fluorescence-Based mAb Aggregation Analysis Solution.
Agilent.com. Retrieved May 15, 2024, from
https://www.agilent. com/en/product/molecular-spectroscopy/fluorescence-spectroscopy/fluorescence-accessories/high-throughput-fluorescence-based-mab-aggregation-analysis-solution
2: Clark, J. (n.d.). The Beer-Lambert Law.
Chem.Libretexts.org. Retrieved May 15, 2024, from
https://chem.libretexts.org/Bookshelves/Physical_and_Theoretical_Chemistry_ Textbook_Maps/Supplemental_Modules_(Physical_and_Theoretical_Chemistry)/Spectroscopy/Electronic_Spectroscopy/Electronic_Spectroscopy_Basics/The_Beer- Lambert_Law
About the author
Ursula Tems has worked at Agilent Technologies since 2011 in Marketing and Product Management. Ursula is part of the Molecular Spectroscopy business, working on product and portfolio management and strategic planning with a focus on the Pharma and Biopharma end markets. With this experience Ursula is involved in new product design, development, and commercialisation.
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www.labmate-online.com/article New innovations in gas analysis technology
Hiden Analytical introduces the QGA 2.0, the latest innovation in gas analysis technology, boasting an array of new features that redefine ease of use, efficiency, and performance. This next-generation gas analyser is designed to meet the evolving needs of both academic and industrial researchers.
The QGA 2.0 presents several new features to enhance user experience and analytical capabilities. Its lightweight and compact design significantly reduce footprint and weight, facilitating easier handling and installation. A streamlined startup process simplifies operation, with a single button managing both pump down and software start.
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More information online:
ilmt.co/PL/Qmzy 62179pr@reply-direct.com Data analysis and interpretation
UV-Vis: • Baseline correction: Subtract baseline absorbance to remove noise.
• Quantitative analysis: Use calibration curves to convert absorbance to concentration.
Fluorescence:
• Correct for inner filter effects: High concentrations absorb excitation light, affecting emission. Apply correction factors.
• FRET efficiency calculations: Understand FRET efficiency based on donor and acceptor fluorescence.
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