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Proteomics, Genomics & Microarrays


Rapid Protein Labelling Kit Launched


Fluidic Analytics Ltd has launched a time-saving and affordable protein labelling kit - fluidiphore rapid amine 503. Absorbing at 503 nm, the new kit can be used with a multitude of techniques including the company’s groundbreaking Fluidity One-W instrument for protein interaction analysis.


Fluorescent labelling of proteins such as antibodies, DNA and lipids is widely used to investigate and better understand protein structure and function. There are various fluorescent dyes available on the market, most of which require a long labelling process over several hours and a purification step to remove excess dye.


Fluidic Analytics’ fluidiphore labelling kit is extremely rapid, taking just 30 minutes to bind proteins rather than four hours to overnight, as is required by other kits. In addition, the fluidiphore kit uniquely does not require a purification step. This is due to a clever ‘triple-lock’ against background effects. Firstly, the absorbance of the dye shifts from 612 nm (free) to 503 nm when conjugated, meaning that unbound dye will not fluoresce at the same excitation wavelength. Quantum yield (the ratio of fluorescence emission to absorption) also increases upon conjugation meaning that unbound dye will have a much weaker signal than bound. Thirdly, any unused dye hydrolyses during the labelling process becoming inactive and unable to bind, therefore preventing the shift in absorbance and increase in quantum yield.


In addition, there is also a useful observable colour change from blue to red/yellow which confirms that labelling is taking place, giving researchers additional confidence in the process.


The fluidiphore rapid amine 503 labelling kit is ideal for use with Fluidic Analytics’ Fluidity One-W instrument to study proteins such as membrane proteins, multi-protein complexes, and intrinsically disordered proteins. The Fluidity One-W enables researchers to accurately assess on-target protein interactions in solution, even in complex, unpurified backgrounds such as crude lysates or blood plasma. The instrument reports absolute size of bound and unbound species, stoichiometry and the nature of binding can be assessed at the same time as binding affinity, giving a complete picture of binding events.


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Unique Electronic Pipetting Range Simplifi es Leukaemia Research at the Cancer Institute


Researchers at the Cancer Institute, University College London, are using Integra’s unique electronic pipette range to simplify their workflows. Yvette Hoade, a research technician in the Payne Laboratory, Department of Haematology, is investigating mutations associated with various blood diseases such as bone marrow failure and myelodysplastic syndrome (MDS), both age-related disorders that greatly increase the risk of developing leukaemia.


She explained: “We’ve started a new DNA extraction project with the aim of quickly going from blood to genotype for 6,000 samples. The multichannel VOYAGER adjustable tip spacing pipette and ASSIST PLUS pipetting robot are a fantastic combination, performing hands-free transfer of blood from collection tubes to 96 well plates, saving hours of time and reducing the risk of cross-contamination. We have three different sizes of pipette - covering volumes from 0.5 to 1,250 µl - so we can use the ASSIST PLUS system throughout our workflow, from DNA extraction to NGS library preparation. The system is also easy to program, making automation accessible to all members of the lab.”


“This automation has improved our throughput, gives us the confidence that the level of accuracy is high, and cuts out the inevitable repetitive aspect of lab work that comes with handling such large sample numbers. The ASSIST PLUS is the ideal lab partner – it’s a solid member of the team now.”


More information online: ilmt.co/PL/5aq6 52040pr@reply-direct.com


Fast and Effective Removal of Interfering Proteins


Incorporating a novel dual hydrophobically treated matrix filter, Microlute™ Protein Precipitation Plate from Porvair Sciences set a new standard for labs looking to remove interfering proteins from serum, plasma or even whole blood samples prior to analysis by Liquid Chromatography/ Mass Spectrometry (LC/MS).


Biological samples commonly contain proteins that interfere with downstream applications such as LC/MS analysis. Microlute™ Protein Precipitation Plates (PPP) use the CRASH method, in which protein is denatured with acetonitrile and the flocculant filtered out, allowing 96 samples to be processed in parallel.


Based on the industry standard MicroLute™ format, but without the chromatographic sorbent, protein ‘crashes’ out of solution and precipitates directly in each well when acetonitrile is added, thus solving all common problems associated with the CRASH technique of protein clean-up. The novel dual frit, hydrophobically treated matrix used by the Microlute™ PPP means that there is no ‘wetting out’ and leakage of sample before the application of vacuum.


Due to its ease of use, high performance and reliability the Microlute™ PPP is commonly used as the protein precipitation plate of choice in many major pharmaceutical companies worldwide.


Watch a video demonstration of the Microlute™ PP: ilmt.co/PL/Q0Ln More information online: ilmt.co/PL/kGJW


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