29 Proteomics, Genomics & Microarrays Pipetting Solutions for Improving ELISA Workfl ows
ELISAs (enzyme-linked immunosorbent assays) are popular plate-based methods to detect and quantify peptides, proteins, antibodies or hormones. However, they generally involve multiple pipetting steps that are time-consuming and tedious to perform, requiring consistent processing to ensure success. Integra Biosciences offers a range of liquid handling solutions to improve ELISA workflows, designed to make your lab life easier and your results more reproducible.
The VIAFLO 96/384 handheld electronic pipette can vastly improve ELISA workflows by enabling the transfer of samples and reagents into 96 or 384 wells simultaneously. All the steps needed to run the protocol can be saved as a custom program on the instrument, which then guides the user with easy to follow prompts. This streamlines the workflow and eliminates user variability. And for even higher throughput, it can quickly fill multiple plates using the Repeat Dispense mode.
If you’re ready for a more walk-away approach, the ASSIST PLUS pipetting robot can automate the ELISA process - just choose the VIALAB program and steps you need, place the appropriate labware on the deck, and let the robot do all the work. Pipetting settings and assay set- up options can easily be modified in VIALAB, making sure the system is always perfectly suited to your assay. Various sample and tube types, and multiple reagents can also be accommodated on the deck, and the choice of pipetting options is huge – you can mount any of Integra’s 25 electronic pipettes onto the ASSIST PLUS.
These solutions are partnered with Integra’s Low Retention GripTips, perfect for reducing the residual tip volumes when pipetting buffers that contain surfactants, such as Tween 20. This combination of flexibility and automated, optimised processing helps to guarantee the consistency and reproducibility of your ELISAs.
More information online:
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High-Throughput Screening using Luciferase Reporter Cell Lines
Amsbio reports on the positive customer feedback received for their One-Step Luciferase Assay kit recently introduced for quantitation of firefly luciferase activity in mammalian cell culture.
Dr Maja Petkovic, Custom Services Business Unit Manager at Amsbio said: “Initial uptake of this new kit has been strong particularly for high throughput screening applications. Our customers have appreciated that the kit offers not just high sensitivity but also the convenience of a homogeneous, single step protocol. In a recent study, researchers at Excellerate Bioscience were able to quantify agonist and antagonist in vitro pharmacological parameters in a luciferase reporter gene assay using our One-Step Luciferase assay system.”
Luciferase is the general term given to a class of oxidative enzymes that catalyse reactions that give off bioluminescent light. Biological researchers can take advantage of this reaction and use it as a readout for processes where a promoter region from a gene of interest is placed immediately upstream of the coding sequence for luciferase.
The One-Step Luciferase Assay kit combines proprietary reagent buffer and reagent substrate components to form a working solution that contains all the necessary components for simple cell lysis and luciferase quantitation. Designed for flexibility - the kit works well with a variety of common media, delivers a signal output that is stable for more than two hours and does not require a luminometer with injectors.
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New Recombinant Eukaryotic SARS-CoV-2 S1-S2 Spike Proteins for Improved Diagnostic Testing of COVID19
Aalto Bio Reagents have announced the availability of its new range of recombinant SARS-CoV-2 spike (S) proteins for diagnostic test manufacturers, vaccine developers and researchers globally. Among all structural proteins of SARS-CoV-2, the S protein is the main antigenic component that is responsible for inducing host immune responses and neutralising antibodies. This makes it the principal focus of therapeutic and vaccine design for COVID-19.
The S protein of SARS-CoV-2 is located on the surface of the viral particles and is comprised of two functional units, S1 and S2. The N-terminal S1 subunit is responsible for virus–receptor binding and the C-terminal S2 subunit mediates virus–cell membrane fusion. When used solely or in combination with the new insect-derived Nucleocapsid protein (N) (code BM 6424), the S1-S2 chimeric glycoprotein (code BM 6422) has demonstrated excellent reactivity in ELISA for detection of IgA, IgG and IgM antibodies against SARS-CoV-2.
“Patients continue to be screened for the virus by PCR on the frontline,” said Philip Noone, CEO of Aalto Bio Reagents, “however there is an important need for serological tests as well to detect all those mild or even asymptomatic cases that may otherwise be missed. Having diagnostic tests that can work in different healthcare settings will be the critical next step in battling this virus and crucial to their development is the selection of high-quality, raw materials used to design them. Our new S1, S2 glycoproteins and S1-S2 Chimeric proteins, in addition to our E. coli-derived N protein launched in February, have been rapidly developed to meet this urgent need.”
More information online:
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Innovative 2D-Gradient for Enhanced PCR Optimisation
Optimising the annealing temperature to improve PCR results is a routine task, so why not optimise as far as you can? More and more findings indicate that optimisation of the denaturation temperature is worthwhile as well. A high denaturation temperature will harm the enzyme and other biomolecules in your assay. A low denaturation temperature may result in inefficient splitting of the complimentary DNA strands, as is often found with GC-rich templates or templates prone to form ‘hairpin structures’.
Eppendorf’s new 2D-Gradient allows optimisation of the annealing and the denaturation temperature in a single run - taking less time than ever before. This gives you the ideal set of temperatures for your PCR assay - quickly, conveniently, reliably.
Read the Application Note:
ilmt.co/PL/Po2e More information online:
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