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31 Proteomics, Genomics & Microarrays The Next Stage of PCR


The new Mastercycler X50 is the elegant synthesis of fl exibility for research applications and standardisation for routine applications such as food testing etc. A new highly intuitive touch screen concept puts all of those benefi ts always at your fi ngertips.


Speed and enhanced optimisation functions like the 2D-Gradient make the Mastercycler X50 the ideal tool for advanced research in molecular biology. The excellent block temperature control and regulation give rise to the next stage of optimisation, whereas the adaptable user management and profound documentation capabilities give peace of mind to laboratories working or conforming to set standards.


A highly intuitive touch display, low noise levels, low power consumption, and the versatile fl exlid lid concept complete the product to be a powerful yet discrete tool. Up to 10 units can be combined – ideal for high throughput applications or labs with a high number of users running different assays. Should you feel you need more fl exibility or throughput, up to 50 units can be combined in a computer- controlled network.


Optimising the annealing temperature to improve PCR results is a routine task, so why not optimise as far as you can? More and more fi ndings indicate that optimisation of the denaturation temperature is worthwhile as well. A high denaturation temperature will harm the enzyme and other biomolecules in your assay. A low denaturation temperature may result in ineffi cient splitting of the complimentary DNA strands, as is often found with GC-rich templates or templates prone to form ‘hairpin structures’.


Eppendorf’s new 2D-Gradient allows optimisation of the annealing and the denaturation temperature in a single run – taking less time than ever before. This gives you the ideal set of temperatures for your PCR assay - quickly, conveniently, reliably.


Visit us at Stand E2 at Lab Innovations 2017


There is a large variety of cyclers available with different ramp rates published. If you compare the corresponding run times by performing the same protocol using different cyclers, you see that ramp rates do not tell the full story about whether a cycler is fast or not.


The better you can control the reproducibility of your PCR, the easier it is to run it consistently and to document the reproducibility of your workfl ow. Excellent block homogeneity, accuracy and precision as well as regular temperature verifi cation, stringent user management and advanced connectivity are cornerstones of a validated PCR workfl ow. The Mastercycler X50 supports your needs for instrument qualifi cation and method validation with the following features:


• Excellent block homogeneity (0.2ºC at 20–72ºC) • Excellent block accuracy and precision (0.15ºC) • Fast temperature verifi cation possible


• Adjustable verifi cation settings – according to your audit needs • Advanced documentation capabilities


• Adjustable user management – from fl exible to strict


• Transparent performance data 44125pr@reply-direct.com 8329ad@reply-direct.com The Next Stage The new Mastercycler® X50


Speed and the 2D-Gradient for en- hanced PCR optimization make the Mastercycler X50 the ideal cycler for advanced research in molecular bio- logy. The block regulation gives rise to the next stage of PCR reproducibility. The adaptable user management and documentation give peace of mind to laboratories working to set standards.


Eppendorf® , the Eppendorf Brand Design and Mastercycler®


> Innovative 2D-Gradient for enhanced PCR optimization


> Heating rate: up to 10°C/s > Wide selection of blocks from a fast silver block to 384


> Intuitive touch display > Connect up to 10 units to a network


> Small footprint are registered trademarks of Eppendorf AG, Germany. All rights reserved, including graphics and images. Copyright © 2017 by Eppendorf AG.


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