Proteomics, Genomics & Microarrays
New Yellow Fever Virus Recombinant Protein for Diagnostic Testing Launched
Aalto Bio Reagents have announced the availability of its first-to-market recombinant Yellow Fever virus (YFV) protein (code CA 6325) for diagnostic test manufacturers, vaccine developers and researchers globally. This His-tagged, recombinant protein is expressed in HEK293 cells and is derived from strain 17D. YFV, a potentially fatal mosquito-borne flavivirus, is prevalent in tropical and subtropical locations in South America and Africa.
YFV is transmitted to humans mainly by sylvatic mosquito vectors of the genera Haemagogus and Sabethes, but has also been known to be spread by the sinister Aedes aegypti mosquito which is responsible for the current Zika virus epidemic. In humans, the majority of YFV infections are asymptomatic; however approximately 15% of infected patients enter what is known as the toxic phase and this can lead to severe complications such as jaundice, multi-organ failure and even death.
Laboratory diagnosis is generally accomplished by means of serological testing for the detection of antibodies during the postviremic phase of the disease (i.e. from the 5th day since the onset of symptoms). YFV is difficult to diagnose, especially in the early stages, as cross-reaction with other flavivirus infections is common. There are no validated IgM ELISA kits commercially available at present and in order for YFV infection to be confirmed by serologically techniques, a differential diagnosis with other flavivirus infections must be carried out.
Philip Noone, CEO of Aalto Bio Reagents, said “Brazil is currently experiencing its largest YFV outbreak in decades. There is an urgent need for a specific and sensitive serological YFV assay in countries such as Brazil, where co-circulation with other flaviviruses is high. Our industry has an unrelenting requirement for access to the most scientifically proven raw materials, a broader range of flexible controls and faster diagnostics. With our Zika, Chikungunya, Dengue Triplex and now our Yellow Fever solution we are truly building a comprehensive tropical product listing to meet this need. We envisage that our YFV protein will provide the critical element to further diagnostic companies’ research and development of IgG and potentially IgM assays. We will continue to focus on the expansion of our tropical disease products with even more additions in 2017-18 to enable our customers to bring superior, best-in-class diagnostic products to market faster, and aid in the development of life-saving vaccines.”
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Sample Prep Kit Provides Unparalleled Benefi ts Compared to Other Commercially Available DNA/RNA Extraction Systems
Cole-Parmer, a leading supplier of laboratory equipment and reagents, is introducing the Arcis Sample Preparation System, which can extract DNA and RNA for downstream processes such as PCR/qPCR and sequencing in just three minutes. A study conducted by the Institute of Infection and Global Health, University of Liverpool, compared the preparation time, DNA yield, PCR and qPCR results of the Arcis Sample Prep Kit with three other kits. The results showed that the Arcis Sample Prep Kit dramatically accelerates pre-analytical sample prep without compromising the quantity and quality of genomic material.
The study showed that competitor preparation kits’ processing times ranged from 20 minutes to 4 hours as they require timely washing, heating and centrifugation steps in order to extract DNA. However, the Arcis Sample Prep Kit required no further instrumentation and was unrivalled as the fastest extraction kit, with a sample processing time of under three minutes. The kit that produced the highest yields was the most labour-intensive and time consuming, and its high yields were very variable. The Arcis Sample Prep Kit produced either greater or equal DNA yields in comparison to the remaining competitors’ kits, and these yields were obtained in a fraction of the time.
Due to its rapid extraction speed of both DNA and RNA, and overall simplicity, the Arcis Sample Prep Kit will save time and money for labs of all sizes. The Kit can be easily integrated into automated systems, and is also ideal for use with Cole-Parmer’s PCR and qPCR hardware and reagents, such as the pathogen detection kits.
The Arcis Sample Prep Kit is a ready-to-use kit that has the dual capability of extracting both DNA and RNA from a wide range of sample types, requiring as little as 30 µl of whole blood, plasma, urine, bacteria, viruses, and parasites as a starting material. The extraction kit consists of a two-step protocol that is completed in just three minutes, massively simplifying the workflow to provide high quality, PCR-ready templates immediately suitable for downstream molecular investigations.
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Orthogonal Platform Enhances Protein Separation and Characterisation
Postnova Analytics reports on the extended data available through integrating its AF2000 MultiFlow Flow Field-Flow Fractionation (FFF) platform with its SC2000 modular multi- detector Size Exclusion chromatography (SEC) system.
Drawing on over three decades of cutting-edge separations leadership, Postnova has designed an advanced orthogonal system that allows both SEC and FFF separations to be carried out on the same sample within one instrument platform. The result is enabling labs around the world to generate more useful information from even complex proteins, antibodies, polypeptides and polysaccharides.
As both separation techniques in the Postnova 2000 Series FFF/SEC system can use the same optimised detector set - UV, RI and MALS (Multi Angle Light Scattering) realising the enhanced applications potential of the integrated system is achieved with minimal redundancy using an automatic switching value and software upgrade kit.
Offering orthogonally operating separation techniques in same platform, the Postnova 2000 Series FFF/SEC system delivers unmatched diagnostic flexibility across an increased application range. The system can be run with different eluents, pH and wide-ranging temperature conditions making it an ideal platform for the characterisation of natural or synthetic macromolecules.
Postnova FFF technology employs a unique crossflow field in which samples are separated by their dynamic diffusion based on molar mass or particle size. Because of this design and the absence of any stationary phase, separations are performed without exerting shear forces and stress on the molecules.
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