7


The BMSS Annual Meeting Conference


Gavin O’Connor opened the meeting with a statement “BMSS is migrating into the next phase of its existence and I encourage all of the British M.S. community to join us on that journey”.


The format of the scientifi c programme this year was similar to that of previous meetings, with invited and contributed oral presentations and posters, but this year BMSS decided to have all the Special Interest Groups have their own sessions which included keynote speakers.


The meeting commenced with the Maccoll Lecture, honouring the memory of Alan Maccoll, a pioneer of mass spectrometry and a founding father of what is now the BMSS which was presented by Professor Lars Konermann (Department of Chemistry, The University of Western Ontario, London, ON, Canada). The lecture entitled ‘A Journey Through Protein Mass Spectrometry: Enzyme Mechanisms, MD Simulations and Supercharged Proteins’ explained, after he quipped about London, Ontario being famous as the birthplace of Justin Bieber and where Johnny Cash proposed to June Carter, 22 February 1968, how native Electrospray ionisation (ESI) permits the formation of desolvated ions from proteins and protein complexes in solution. Further analysis of these gas phase biomolecules by mass spectrometry shows the nature of proteins. He went on to elucidate a journey through protein mass spectrometry showing both gas phase and solution phase options and explaining native ESI with ‘supercharging’.


His lecture was followed by the welcome mixer and exhibition, which was held in the Floral Hall.


The welcome mixer, enjoyed by all, consisted of red and white wine, nuts and crisps, a venerable feast for the starving Mass Spectrometrists and exhibitors who had been busy all day. Upon the conclusion those that were of a mind and still hungry left promptly at 20:00 for ‘the social event at the Dog Bowl’ a short walk from the conference venue, with the incentive of ‘free food and drinks’ for the 160 arrivals.


The two-day meeting started in earnest on Wednesday with the Robinson Lecture, a lecture to recognise the contribution to Mass Spectrometry of Professor Carol Robinson. The lecture was titled ‘Investigating biodiversity by protein mass spectrometry’ and was given by Professor Peter Roepstorff (Department of Biochemistry and Molecular Biology, University of Southern Denmark).


Wednesday mornings two parallel sessions followed a one hour coffee break which took place after the Robinson Lecture and included the following oral presentations:


Session 1 - Instrumentation Chair: Helen Cooper


Emmanuel Raptakis - Keynote: Mass spectrometry development in industry: from the Mag-TOF to the Omnitrap.


Bruno Bellina - Spectroscopy: Combining Light Activation, Ion mobility and Mass Spectrometry data in a snap #LAIMMS.


Jason Wildgoose - Comprehensive DIA on a Q-ToF instrument for discovery and quantitative analysis utilising a scanning quadrupole.


Ian Sinclair - Acoustic Mist Ionisation- from Concept to Automated High Throughput Platform.


Session 2 - MS Solutions for challenging analytes


Chair: Tony Bristow


Richard Bourne - Keynote: Self- Optimising Flow Reactors for Rapid Process Development.


Christopher Gray - Screening biocatalytic reactions within live bacterial colonies by DESI-(IMS)-MS.


David Clarke - A Systematic Evaluation of the Integration of Ion Mobility into an Online Hydrogen Deuterium Exchange Mass Spectrometry Workfl ow


Christopher Whitmore - Quantifying ion suppression in online reaction monitoring by ESI MS.


Particularly interesting presentations in Session 1 were:


Firstly, Emmanuel Raptakis – Keynote lecture which was dedicated to Peter Derrick (1945 – 2017) and his illustrious career as a pioneer in mass spectrometry and how Peter mentored Emmanuel through his PhD at Warwick University. Emmanuel then went on to describe his own career history and pioneering work in developing the Omnitrap platform (FasmaTech), a unique ion processing system designed to provide access to an arsenal of ion activation-dissociation techniques all embedded in the same unit. The Omnitrap platform can be adapted to existing or custom engineered instrumentation. This unique technology is currently available as a retrofi t to the Q ExactiveTM instrument series (Thermo Fisher Scientifi c) and also with Fasmatech’s oTOF mass analyser.


Secondly Bruno Bellina (Michael Barber Centre for Collaborative Mass Spectrometry, Manchester Institute of Biotechnology, and Photon Science Institute, University of Manchester) discussed how an ion mobility enabled Q-ToF (Waters Synapt G2-S) had been modifi ed to combine ‘action spectroscopy IR/UVPD’. The instrument had the trapping and light activation occurring prior to the IMS cell and utilised a newly developed acquisition and analysis software package called ORIGAMI.


These sessions were followed by a lengthy two-hour lunch break at 12:10, which was held in the exhibition area and permitted attendees time to network with vendors, view posters and attend the Bruker Lunch Seminar entitled ‘From a spectrum to a speed revolution – 25 years of MALDI mass spectrometry’.


Examples of the wide range of challenging chemistries were shown, together with fast method optimisation and the decision-making process, e.g. if not retained or early eluters by RPUHPLC or a HILIC method (polar compounds) then try SFC.


Bob Boughtfl ower (GSK, Medicines Research Centre, Gunnelswood Road, Stevenage) discussed how reverse phase generic gradient (U)HPLC methods of analysis have been adopted across the analytical measurement industry. However, it is often the case where practitioners do not take advantage of the benefi ts of separation speed and performance that is available from modern stationary phase materials and instruments. Bob demonstrated that the performance enhancements that are easily accessible and the choices of instrument and column formats. Examples were shown of ultra-fast separations and where these methods can routinely apply.


This session concluded at 15:40 for a tea break and time to peruse the exhibition and posters.


Session 5 - New Techniques for Ambient Ionisation


Chair: Andy Ray


Stephen Taylor - Keynote: Desorption Atmospheric Pressure Chemical Ionisation (DAPCI) – development and applications.


Julia Abda - Imaging and ex-vivo Tissue Analysis by IR Laser Assisted REIMS – towards an integrated MS-based tissue ID system.


Bryan McCullough - Results of the Second BMSS Ambient Ionisation SIG Interlaboratory Study.


Stephanie Rankin - Development of a direct surface analysis method for in-situ VOC profi ling of biological fl uids.


Session 6 – Analysis of Big Data Chair: Peter O’Connor


Mark Andre Delsuc – Keynote: Tackling the Big-Data problem in 2F FT-ICR-MS.


Remy Gavard - Themis: batch pre- processing for ultrahigh resolution petroleomics data.


Christopher J. Arthur - Data plotting strategies for the visualisation of complex datasets.


Catherine Evans - The SNAP Advantage: Enabling High Resolution Protein Analysis.


A drinks reception and the conference dinner at the Manchester Museum of Science and Industry rounded off a full fi rst day of the BMSS Annual Meeting and Conference.


Session 3 - Imaging Chair: Malcolm Clench


Josephine Bunch - Keynote: Developing A Google-Earth View Of Tumour Metabolism Through Multiscale Molecular Imaging.


Renata Soares - Tissue microarrays analysis using DESI-MSI.


Amaia Carrascal Minino - Imaging oxidative damage in irradiated HCT-116 spheroids using DMPO.


Cristina Russo - Method development for quantitative investigation of Terbinafi ne hydrochloride in a 3D skin model by MALDI-MSI


Session 4 – Innovative Separations Chair: Julie Herniman


John Langley - Keynote: Supercritical Fluid Chromatography - Mass Spectrometry: The Final Piece in the Hyphenation Jigsaw.


Bob Boughtfl ower - The ultimate speed and performance available from Generic Gradient methods – what does this mean for future practice?


Lewis Couchman - Opportunities for ultra-rapid LC-MS/MS in high-throughput bioanalysis.


Maria A. Van Agthoven - Two-dimensional mass spectrometry in a linear ion trap.


The two parallel Wednesday sessions continued after the lunch break with sessions on: Of interest in these sessions were:


John Langley (University of Southampton, Chemistry, Southampton) reported on how SFC now delivers on the chromatographic promises with robust and reliable instrument platforms available from several manufacturers. The solvation power, selectivity and peak capacity of modern Ultrahigh Performance Supercritical Fluid Chromatography (UHPSFC) coupled with mass spectrometry provides the ideal analytical platform to address classes of compounds that were previously a challenge for other chromatographic methods. UHPSFC complements existing liquid chromatography and gas chromatography options (HPLC-MS (ESI/APCI), GC-MS (EI/CI) and provides a comprehensive, modern and fast separation science with the mass spectrometry option to address the wide range of diverse and challenging chemistries. UHPSFC-MS sits between LC-MS and GC-MS affording extended capability and complementarity and delivers unique solutions across a broad range of application areas, e.g. synthetic organics, pharmaceuticals, petrochemistry, lipids, nucleotides and many other areas.


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