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LABORATORY PRODUCTS Beauty to Rival Swarovski
Greiner Bio-One GmbH has developed a new platform for the production and analysis of protein crystals. CrystalSlide for counter diffusion applications enables crystals to be analysed in an X- ray source and is also suitable for applications with polarised and UV light.
A key prerequisite for targeted drug development is knowing the three-dimensional structure of proteins. In order for this to be possible, however, the proteins must be available in the form of high-quality pure crystals. Producing and analysing these is very time- consuming and thus expensive.
Greiner Bio-One GmbH has now developed a new platform for counter diffusion applications – that is to say for the production of protein crystals in a concentration gradient – in a handy slide format. CrystalSlide has twelve microstructured channels. The design has been optimised to ensure that the inlets are suitable for manual filling using a pipette and the slides can be used in automated systems – in conjunction with a frame in standard microplate format.
The benefits of CrystalSlide are not limited to the production of protein crystals. In addition to enabling its valuable contents to be analysed under any conventional microscope, CrystalSlide is also suitable for all commonly used automated imaging systems. At just 0.6 millimetres thickness, the polyolefin slides combine excellent optical characteristics with low birefringence and are permeable to UV light and X-rays for in-situ analysis of protein crystals. The low sample volume required – just 600 nl – also helps cut the cost of protein crystallisation processes significantly. CrystalSlide™ is part of Greiner Bio-One GmbH’s CrystalStar™ family, which includes a number of protein crystallisation platforms and associated accessories.
Circle no. 357 Accelerating Sample Preparation
Porvair Sciences Ltd has produced an application note that describes a method for the rapid and sensitive determination of a novel drug entity in human plasma. Key to the high throughput of this method is the use of the Microlute™ 96-well SPE system, which allows a batch of samples to be prepared for injection in less than half the time that is actually required to analyse them. The method meets all regulatory requirements for clinical studies, and is suitable for the analysis of large numbers of samples. A copy of Application Note D may be downloaded from the Porvair website.
The developed method using the Microlute™ was validated over a concentration range of 100 to 10,000 pg /ml. Recovery using the SPE method was approximately 60%, and consistent across the calibration range. The authors describe how the Microlute™ SPE method, unlike protein precipitation sample preparation, is free from LC/MS matrix effects that may influence the ionisation of the compound(s) of interest.
Developed in conjunction with a leading pharmaceutical company the Microlute™ has become the benchmark for high throughput sample preparation across a range of applications. The innovative design of the Microlute™ from Porvair Sciences Ltd offers all the advantages of automated and high throughput SPE sample preparation in a convenient microplate format capable of rapidly processing 96 samples in one go repeatedly and precisely.
Circle no. 359 Library Maintenance for the Smaller Pharma
An application note from Genevac describes how their Model DD-4X centrifugal evaporator has been used by a pharmaceutical company to address the sample integrity and physical storage problems associated with compound library maintenance.
Entitled ‘Library maintenance for the smaller pharma’ the application note details a high throughput process to produce compound libraries as dry films that have been validated for long-term storage. The authors describe how compounds were solubilised, reformatted from 96- to 384-well plates and then dried down using a Genevac DD-4X centrifugal evaporator for storage at room temperature. The high capacity of the Model DD-4X is shown to facilitate sample-safe evaporation of sixteen 384-well plates to dryness in only 25 minutes at 40◦
C. The described dry film methodology reduces the time for compound
preparation and produces enough assay ready sets of dry film plates for at least 10 screens. The Model DD-4X from Genevac is an affordable, high- performance centrifugal evaporator system designed to handle the wide range of sample types and formats encountered in pharmaceutical laboratories.
Circle no. 360 Circle no. 358
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