18 May / June 2014


the current commercial version of ACD/ LC Simulator (version 2014). It is thereby possible to accurately model and optimise protein separations based on both RPC and IEC. It should, in principle, also be possible to model HILIC and HIC (Eqns. 3 and 1 or 2 respectively [2]) although this has not been evaluated using ACD/LC Simulator.


4. References


[1] Evaluate Pharma, World Preview (2013), Outlook to 2018—Returning to Growth, 2013. June, p. 13.


[2] M.A. Stadalius, H.S. Gold, L.R. Snyder, J. Chromatogr. A, 327 (1985) 27.


[3] L.R. Snyder, J.W. Dolan, High- Performance Gradient Elution: The Practical Application of the Linear-


Solvent-Strength Model, Hoboken, NJ: Wiley, 2007, pp. 228.


[4] P. Jandera, J. Chromatogr. A, 1126 (2006) 195.


[5] R.I. Boysen, A.J.O. Jong, M.T.W. Hearn, J. Chromatogr. A, 1079 (2005) 173.


[6] A.W. Purcell, G.L. Zhao, M.I. Aguilar, M.T.W. Hearn, J. Chromatogr. A, 852 (1999) 43.


[7]M.T.W. Hearn, G.L. Zhao, Anal. Chem., 71 (1999) 4874.


[8]S. Fekete, S. Rudaz, J. Fekete, D. Guillarme, J. Chromatogr. A, 70 (2012) 158.


[9]ACD/LC Simulator, version 12 (12.02), Advanced Chemistry Development, Inc., Toronto, On, Canada, www.acdlabs.com, 2013.


Expanded Availability of Chromosorb GC Packings


Agilent Technologies Inc. today announced the availability of Chromosorb W support materials for packed GC columns. Chromosorb W is a solid support used in the separation of polar and a-polar compounds. With the addition of the Chromosorb W materials, Agilent now supplies the full line of Chromosorb W, P, G, T and 750 Series supports and the Century Series 101, 102 and 103 packings.


“Following a nearly two-year supply shortage of Chromosorb W, we are very pleased to provide laboratories with these vital support materials, which are now in stock and can be ordered immediately,” said Mike Feeney, vice president and general manager of Agilent’s Supplies Division.


Agilent J&W packed GC columns are designed and manufactured to provide the highest quality and reproducibility for all sample types associated with packed column separations. They are used in a wide variety of applications in hydrocarbon processing, petrochemical, pharmaceutical raw materials analysis, and quality control.


For more information, please visit www.agilent.com/chem/packedcolumns.


[10] M.A. Stadalius, H.S. Gold, L.R. Snyder, J. Chromatogr. A, 296 (1984) 31.


[11] G. Vanhoenacker, P. Sandra, J. Chromatogr. A, 1082 (2005) 193.


[12] L.R. Snyder, J.W. Dolan, High- Performance Gradient Elution: The Practical Application of the Linear- Solvent-Strength Model, Hoboken, NJ: Wiley, 2007, pp. 399.


[13] M.R. Euerby, F. Scannapieco, H.-J. Rieger, I. Molnar, J. Chromatogr. A, 1121 (2006) 219.


[14] B.F.D. Ghrist, B.S. Cooperman and L.R. Snyder, J. Chromatogr. A, 459 (1988) 1.


[15] N. Lundell, J. Chromatogr. A, 639 (1993) 97.


[16] J.D. Stuart, D.D. Lisi, L.R. Snyder, J. Chromatogr. A, 485 (1989) 657.


Technical Note Compendium for Solid-Core HPLC Columns


Thermo Fisher Scientific has released a new 58-page compendium for its solid-core HPLC columns (Thermo ScientificTM AccucoreTM


HPLC columns) titled, Solid Core Technical Compendium. These solid-core HPLC columns use our Core Enhanced TechnologyTM


to produce a 2.6 µm solid-core material


with a very tight particle size distribution. The particles in the columns are not fully porous but instead have a solid silica core surrounded by a porous outer layer. The very tight particle size distribution results in columns with high permeability. Therefore, bar for bar, the columns produce improved separation efficiency when compared to fully porous materials. The columns are suited for biopharma, pharmaceutical, environmental, bioanalysis, and food and beverage applications.


The compendium technical notes cover the following topics: an overview of the core enhanced technology used in the columns that enable fast, high efficiency HPLC analysis plus the theory and benefits of solid core particles; pressure factors in the columns; comparison of reversed-phase selectivity of the columns; comparison of column performance and impact of particle diameter; and, comparison of chromatographic resolution of solid core columns to fully porous columns.


For more information visit www.thermofisher.com


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