13
Table 1. List of column combinations used in 2D LC and their application areas Combination of Chromatography Modes Columns Used
RP x RP RP × RP
RP × RP
RP-temperature gradient interaction Chromatography (RP-TGIC)×RP (LC–CC)
NP x RP NP × RP Microbore
RP × NP (HILIC) RP x IEC IEC (SAX) × RP
NP x SEC NP
(LC–CC) × SEC RP x SEC
Affinity (titania) × RP CN×monolith C18 Mobile phases Used
Gradient methanol × stepped ACN
HS-F5 × carbon clad zirconia Gradient ACN × gradient ACN
C18 × C18 silica×monolithic C18
C18 Microbore×aminopropyl silica
RP×IEC (SCX) Isocratic DCM/ CAN
Gradient n-hexane and EtOH × gradient IPA, ACN, water
Gradient ACN × isocratic EtOH – DCM, water
Gradient ACN (TFA)×gradient ACN (phosphate, KCl)
Quaternary amine-SAX×C18 Stepped guanidine thiocyanate salt × gradient ACN
Silica×HSPgel-RT, MB-L/M Chloroform/diethylether × chloroform
SEC×RP G2000SWXL×BDS-C18
Titania column × monolith C18
References
1. J. Zhang, D.Y. Tao, J.C. Duan, Z. Liang, W.B. Zhang, L.H. Zhang, Y.S. Huo, Y.K. Zhang, Anal. Bioanal. Chem. 386 (2006) 586.
2. D.R. Stoll, J.D. Cohen, P.W. Carr, J. Chromatogr. A 1122 (2006) 123.
3. K. Im, Y. Kim, T. Chang, K. Lee, N. Choi, J. Chromatogr. A 1103 (2006) 235.
4. P. Dugo, V. Skerikova, T. Kumm, A. Trozzi, P. Jandera, L. Mondello, Anal. Chem. 78 (2006) 7743.
5. P. Jandera, J. Fischer, H. Lahovska, K. Novotna, P. Cesla, L. Kolarova, J. Chromatogr. A 1119 (2006) 3.
Next-Generation UHPLC Autosampler
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Other new features of the 1290 Infinity Multisampler include a dual-needle injector set-up that enables optimal injection speed, as well as unparalleled flexibility for injections, all within a single instrument. New software features improve workflow efficiency and enable easy data-entry of large sample sets. The product was unveiled at the HPLC-2014 conference in New Orleans, Louisiana, shipment of new orders is scheduled within a few months.
For more information visit
www.agilent.com 6. L.A. Holland, J.W. Jorgenson, J. Microcolumn Sep. 12 (2000) 371.
7. L. Coulier, E.R. Kaal, T. Hankemeier, J. Chromatogr. A 1070 (2005) 79.
8. G.J. Opiteck, J.W. Jorgenson, M.A. Moseley III, R.J. Anderegg, J. Microcolumn Sep. 10 (1998) 365.
9. G.J. Opiteck, S.M. Ramirez, J.W. Jorgenson, M.A. Moseley III, Anal. Biochem. 258 (1998) 349.
10. K. Hata, H. Morisaka, K. Hara, J. Mima, N. Yumoto, Y. Tatsu, M. Furuno,
11. N. Ishizuka, M. Ueda, Anal. Biochem. 350 (2006) 292.
Water (TFA) × gradient ACN (TFA)
Gradient potassium phosphate × gradient acetonitrile (TFA)
Application Areas Pharmaceutical
Metabolomics
Ref. 1
2 Branched polystyrenes 3
Essential oils and carotenoids
Polymers Proteomics Peptides
Polymer degradation Studies
Peptides Phosphopeptides 4 5 6 7 8 9, 10 11
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