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September 2009


also be considered. This utilises the XBridge HILIC column in 100% aqueous (potentially with EtOH gradient and CO2 to make it EFC).


SFC was in more prominence too. Eric Lesellier’s group have been studying the fundamentals of SFC including measuring the apparent pH in the mobile phase (CO2:MeOH, pHapp <5) and chiral recognition mechanisms with a variety of CSPs. Whilst Claudio Brunelli (Pfizer, UK) gave an excellent talk on QbD in achiral pSFC method development. This covered the screening of stationary phases, mobile phases, acid/base additives.


Exhibition;


One of the high profile events during the exhibitionwas the launch of the newAgilent 1290 Infinity UHPLCsystem. Such is the interest in UHPLCthese days ~10 vendors of suitable instrumentation, plus others offering consumables specific to thismarket segment exhibited.


Many vendors were showcasing new columns – in particular sub 2um and HILIC phases. It was noticeable that a lot of vendors were keen on transferability ofmethods fromUHPLC to HPLC and as such having consistency across particle size (a poster byMallard, Clarke, Petersson andNightingale describes this benefit very well).


Other interesting hardware included Dionex’s universal finger tight Viper fittings (up to 1000bar), VWR’s EcoPure columns interestingly use a porous filminstead of a frit, and the Aurora A5 SFC fusion – a module compatible with an Agilent 1100 (or later) that allows SFC on conventional LC systems.


The vendor seminars reflected the above. There was also representation fromBischoff Chromatography with their POP-LC, this is fascinating tool if a bit odd…almost LC for the LEGOgeneration! They now have moved in to gradient applications, whichmaymean that it ismore applicable to Pharma R&D.


Additionally the release of a new Agilent CE did not go unnoticed, and there has been a small resurgence in CE owing to its improved sensitivity vs. older instruments, “green” nature and advantages for largermolecules. Part of the increased use has been down to improving the ease of use with several “platforms” and kits/applications solutions fromAgilent and Beckman.


Space limitations prevent a more exhaustive review of exhibited products but a visit to many of the vendors’ web sites from the organiser’s web site, www.hplc2009.org will yield more information. It would also be remiss not to mention the fact that Issue 6 of Chromatography Today (editorial theme Bioanalysis) distributed at the meeting, was well received by people unfamiliar with the magazine resulting in many more readers requesting future copies.


Posters;


There were lots of posters, all on the key topics. It is recommend that browsing the abstracts is the best way to find posters of specific interest or author. Several posters were dedicated to tips, tricks and issues relating to UHPLC


Unsurprisingly there were many posters from vendors (Waters, Varian, Grace being most notable), these should be available to look at on their respective websites.


List of poster winners Agilent Technologies Sponsored "Best Poster Award"


1st prize to S. Khirevich, University of Marburg/Germany: “Quantitative Three-Dimensional Structure-Transport Analysis in Chromatographic Beds of Arbitrary Cross-Section”


2nd prize to I. Francois, Exxon Mobile, Mechelen/Belgium: “Comprehensive and Off-Line Supercritical Fluid Chromatography - Reversed Phase Liquid Chromatography for the Analysis of Complex Triglyceride Profiles”


Fig 7. Social Networking Dresden Style at the HPLC 2009 Symposium Dinner and Party at BallhausWatzke. From Left to Right- Gräfin Kosel” (engl. Countess Kosel), the mistress of Saxony’s King August The Strong Professors Barry Karger, Christian Huber and. Karl- Siegfried Boos, and: “August der Starke” (engl. August the Strong), King of Poland and King of Saxony


©(kBics Photographers) Dates of next meetings


The onerous tasks to ‘follow that’ falls upon the Organising committee for the 2010 event – Dr Steven Cohen will chair, and that will be held in Boston, MA USA between June 19-24th (www.hplc2010.org). The meeting then returns to Europe and will be held in Budapest, Hungary (June 19-24th, 2011) and chaired by Professor Attila Felinger. More details can be found at www.hplc2011.org .


3rd prize to M. Haugg, University of Ulm/Germany: “Star-like Poly (alkyleneoxide) Coated Open Tubular Columns for Tuning the Separation Mode in Liquid Chromatography”


with honorary mentions in the Best Poster Award Competition to:


K. Broeckhoven, Free University of Brussels/Belgium: “Variability of Column Temperature During Gradient Separations in Current and Future Very High Pressure Liquid Chromatography Systems”


and


F. Vilaplana, University of Amsterdam/The Netherlands: “Towards New Polymeric Supports for Amylose Starch Separations”


Pfizer Sponsored Best Poster Award for “Innovation in Pharmaceutical Analysis”


1st prize to R. Nicoli, University of Geneva/Switzerland: “Development of an In-Capillary Method to Nanoscale Automated In Vitro Cytochromes P450 Assays”


2nd prize to P. Kacer, Institute of Chemical Technology, Prague/Czech Republic: “Immunomagnetic Molecular Probe for Monitoring of Biomarkers Present in Exhaled Breath Condensate”


four third prizes to:


B. He, Bristol-Myers Squibb, New Brunswick/USA: “Chiral Separation of Pharmaceutical Compounds with Multiple Chiral Centers by Liquid Chromatography: Method Development Strategy and Application of Pre-Column-Derivatization”


D. El Zeihery, University ofWuppertal/Germany: “Highly Variable Epigenomes in Healthy Adult Clones”


L. Steinhauser, University of Tübingen/Germany: “Identification of Flavonoids in Leaves of Graptophyllum pictum by High Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry”


S. Lackner, University of Innsbruck/Austria: “Detection of Hypoxia in Cultured Mammalian Cells in Vitro by HPLC-ECD”


And finally…….


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