40
September 2009
Report on 2009 Spring Symposium of the Chromatographic Society
by BernieMonaghan, Editor for Separations Science and Spectroscopy, International Labmate, email:
bernie@intlabmate.com DeniseWallworth, Hon, Secretary, Chromatographic Society, email:
denise.wallworth@sial.com
It is the scientific argument equivalent of ‘Which came first, the chicken or the egg?’ only in this case it is ‘Liquid Chromatography or Spectroscopy, primarily Mass Spectroscopy?’ One could debate the criteria as to which form of each we are voting on but the premise remains the same as is the inescapable fact that the two do actually have a symbiosis and exist to each others mutual benefits on an increasing level especially at the life sciences end in the pharmaceutical industry.
The spring symposiumof the Chromatographic Society debated this inter dependency in a fascinatingmanner with well practised speakers in the tandemfield of LC- (almost any spectroscopic technique but primarilyMass) in a two daymeeting entitled ‘Advances in LC/MS and Related Hyphenated Techniques’ held in Sunderland, UK during May 13/14th. Alongside vendors, some of whommade interesting presentations, almost 80 interested participants attended fromthe UK and Europe.
Figure 2: Success Rates of Lux™Phases in RP for over 200 Racemates (Rs>1.5) – Various Conditions
Presenter and Title Gold sponsor seminar: Dr Stefan Schuette (Agilent, UK) – Infinitely Better LC for LC/MS
Vendor 2: Dr Luisa Pereira (Thermo Fisher Scientific, UK) – Evaluating the sensitivity of sub 2 m particle packed columns for the LC/MS analysis of complex biological samples
Vendor 3: Dr Khalil Divan (Dionex, UK) - Ion Exchange-MS Application for Ionic and Polar Compounds
Vendor 4: Dr Jackie Mosely (Durham University on behalf ofWaters, UK) – Practical applications of an Atmospheric Pressure GC (APGC) ion source and an Atmospheric Solids Analysis Probe (ASAP)
Vendor 5: Dr Jonathon Nielson (ACDLabs, UK) - Data Overload! High- Throughput Data Reduction of Complex Datasets.
Vendor 6: Pauline Leary (Smiths Detection, USA) - Ion Mobility Spectrometry for the Identification of Microbiological Samples
Vendor 7: Peter Ridgway (TharSFC, UK) – Interfacing SFC with MS Table 1. Vendor Presentations
LC and MS are now inseparable and integral to the pharma industry, according to the first keynote speaker, Dr Frank Pullen, who gave a personal view of the historical background to techniques hyphenated to MS. After struggling for many years to link GC to MS, scientists can now link MS to virtually any, including SFC. The first MS, based on moving belt technology, could only be used in normal phase, but the invention of APCI using a simple ionisation source in 1987 was the real turning point. Advances in instrumentation and automation mean that non-MS experts can utilise the technique in bench-top, reliable systems. SFC-MS is an interesting development in that greater ionisation is achieved without using a voltage, giving greater sensitivity. In Dr Pullen’s words, ‘it has shunted NMR sideways’. Latest applications for LC-MS include the detection of anti-sense strands after including a drug into RNA duplexes – a novel drug delivery process. Further development in this area now lies more in the chromatography than the MS detection. GC-MS and Metabolomics are being used in the search for biomarkers. The future for MS will include reaction monitoring at a miniature level to enable chemists to
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