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A concern still remains though when analysing and quantifying in the same run several labile compounds of both related and unrelated structures. Even when using MS/MS, chromatographic separation has to become a crucial component to raise assay quality. The recent implementation of Ultra High Pressure Liquid Chromatography in most bioanalytical labs has not only generated a significant increase in sample analysis throughput, but has also allowed high chromatographic resolution to come back into play in a field where the typical 5 cm chromatographic columns were merely used for peak refocusing. Coupling an on-line extraction approach allowing minimal sample preparation with a powerful analytical separation has to be the ultimate approach for bioanalysis. The new Transcend
platform [12] is coming very close to provide an off-the-shelf technical solution to achieve just that.With Transcend, the analytical compartment of the typical TurboFlow platform has been upgraded with high pressure flux pumps able to deliver UHPLC resolution post extraction. Despite limited data available in the literature and the need for specialist hands to operate it, this one-size-fit-all platform could become an answer to emerging bioanalytical challenges.
References 1. Quinn, H.M.; Takarewski, J.J. Int. Patent Number WO97/16724, 1997.
2. Chassaing, C.; Luckwell, J.;Macrae, P.; Saunders, K.;Wright, P.; Venn, R.; Chromatographia (2001), 53(3/4), 122-130.
3. C.A. Lipinsky, F. Lombardo, B.W. Dominy, P.J. Feeney, Advanced Drug Delivery Reviews, 23 (1997) 3-25.
4. P. Turnpenny, D. Fraier, C. Chassaing, J. Duckworth, J. Chromatogr. B, 856 (2007) 131-140.
5. M. Berube, Application note: 458, Thermo Scientific (
www.thermo.com/appnotes)
6. C. Chassaing, H. Stafford, J. Luckwell, J. Fane, A.Wright, A. Edgington, Chromatographia, 62 (2005) 17-24.
7.
P.Mora, U. Ceglarek,
F.Manzotti, L. Zavota, A. Carta, R. Aldigeri, J.G. Orsoni, Graefes Arch. Clin. Exp. Opthalmol, 246(7) (2008) 1047-1052.
8. A. Hartmann, R. Krebber, G. daube, K. Hartmann, J. Vet. Pharmacol. Ther., 31(2) (2008) 87-94.
9. W. Zeng,
D.G.Musson, A.L. fisher, L. Chen,M.S. Schwartz, E.J.Woolf,
A.Q.Wang, J. Pharm. Biomed. Anal., 46(3) (2008) 534-542.
10.R. Krebber, F.J. Hoffend, F. Ruttmann, Anal. Chim. Acta., 637 (2009) 208-213.
11.J.L. Zhou, J.J. An, P. Li, H.J. Li, Y. Jiang, J.F. Cheng, J. Chromatogr. A., 1216(12) (2009) 2394-2403.
12.P. Ashton, A. Allen, B. Ardrey,
S.McDonnell, S. Robinson, Application note: 461, Thermo Scientific (
www.thermo.com/appnotes)
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