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UHPLC Enables Enhanced Resolution Separations of Complex DrugMixtures
Thermo Fisher Scientific (UK) has issued a new application note to demonstrate superior ultra high performance liquid chromatography (UHPLC) capabilities for the separation of drugs of abuse in complex drugmixtures. Illustrated in the application note is the advanced ability of the Thermo Scientific Accela UHPLC systemto performefficient, improved resolution separations using a unique quaternary UHP mixing pump. The new application note, entitled “A Comparison of Binary and TernaryGradients Using Stage 1 Illicit Drugs,” is available for download via
www.thermo.com/accela.
When using an HPLCmethod to separate a complex drugmixture, a simple binary solvent gradient can often fail to provide sufficient resolution of all analytes. In order to improve the resolution, the separation factor has to bemanipulated by using a third solvent. However, this approach is not possible with typical high-pressure binary solventmixing equipment. The Accela UHPLC systemovercomes this limitation, featuring a newly introduced quaternarymixing pump that facilitates the use of up to four solvents for convenientmethod development, systemflushing and eluent preparation. Changing of solvents, buffers and pH values is quick and easy, greatly simplifying themethod development process.
The new application note describes an effectivemethod for the separation of drugs using the Accela UHPLC in conjunction with the Thermo ScientificMSQPlusMass Detector, a fast-scanning single quadrupolemass spectrometer (MS). Amixture of 14 drugs is separated and baseline resolved by UHPLC/MS within eightminutes on a Thermo Scientific Hypersil GOLD PFP column using a ternary solvent gradient, which is not possible using a conventional binary solvent
gradient.Methanol was found to provide improved resolution formost of the drug compounds compared with other eluents. Formore information email
analyze@thermofisher.com.
Anatune expands into HPLC with new £1/3 million laboratory
Anatune continues to grow with its entry into the High Performance Liquid Chromatographymarket. The company has recently celebrated the opening of a new £1/3 million HPLC application development laboratory and has recruited Paul Roberts, formerly of the Centre for Environment, Fisheries & Aquaculture Science, as HPLC applications chemist.
Paul Roberts joins the company after eight
years at CEFAS, where he was involved in a variety of projects focused on the development of analyticalmethods (LC & GC-MS) for the determination and identification of emerging contaminants and hazardous substances in the aquatic environment. He previously worked at Cranfield University.
Anatune is supported in its HPLC development by both Agilent Technologies and GERSTEL GmbH. Both companies have made significant investments in new HPLC products.
Anatune’s Technical Director, Bob Green, said: “The success of our Gas Chromatography business is based upon our ability to provide complete solutions to UK specific needs. Our customers have encouraged us tomake thismove into HPLC in the expectation that we will fulfil a similar role here. Much of our experience in automation and productivity enhancement for GC and GC-MS translates directly to LC and LC-MS, so I amconfident that we have a great deal to offer.” Formore information visit
www.anatune.co.uk.
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